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Reference
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| Citation |
Konev, A.Y., Yan, C.M., Acevedo, D., Kennedy, C., Ward, E., Lim, A., Tickoo, S., Karpen, G.H. (2003). Genetics of P-element transposition into Drosophila melanogaster centric heterochromatin. Genetics 165(4): 2039--2053. |
| FlyBase ID |
FBrf0167658 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
14704184 |
| PubMed Abstract |
Heterochromatin is a major component of higher eukaryotic genomes, but progress in understanding the molecular structure and
composition of heterochromatin has lagged behind the production of relatively complete euchromatic genome sequences. The introduction
of single-copy molecular-genetic entry points can greatly facilitate structure and sequence analysis of heterochromatic regions
that are rich in repeated DNA. In this study, we report the isolation of 502 new P-element insertions into Drosophila melanogaster
centric heterochromatin, generated in nine different genetic screens that relied on mosaic silencing (position-effect variegation,
or PEV) of the yellow gene present in the transposon. The highest frequencies of recovery of variegating insertions were observed
when centric insertions were used as the source for mobilization. We propose that the increased recovery of variegating insertions
from heterochromatic starting sites may result from the physical proximity of different heterochromatic regions in germline
nuclei or from the association of mobilizing elements with heterochromatin proteins. High frequencies of variegating insertions
were also recovered when a potent suppressor of PEV (an extra Y chromosome) was present in both the mobilization and selection
generations, presumably due to the effects of chromatin structure on P-element mobilization, insertion, and phenotypic selection.
Finally, fewer variegating insertions were recovered after mobilization in females, in comparison to males, which may reflect
differences in heterochromatin structure in the female and male germlines. FISH localization of a subset of the insertions
confirmed that 98% of the variegating lines contain heterochromatic insertions and that these schemes produce a broader distribution
of insertion sites. The results of these schemes have identified the most efficient methods for generating centric heterochromatin
P insertions. In addition, the large collection of insertions produced by these screens provides molecular-genetic entry points
for mapping, sequencing, and functional analysis of Drosophila heterochromatin.
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
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Published In
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| Abbreviation |
Genetics |
| Title |
Genetics |
| Authors |
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| Volume range |
1- |
| Year range |
1916- |
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| Place of publication |
Austin, TX, etc |
| Language of publication |
English |
| ISBN/ISSN |
0016-6731 |
| CODEN |
GENTAE |
Data from Reference
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Aberrations (3)
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Alleles (1)
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Constructs (1)
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Genes (1)
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Insertions (6)
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