Reference Report

Reference
Citation	Konev, A.Y., Yan, C.M., Acevedo, D., Kennedy, C., Ward, E., Lim, A., Tickoo, S., Karpen, G.H. (2003). Genetics of P-element transposition into Drosophila melanogaster centric heterochromatin. Genetics 165(4): 2039--2053.
FlyBase ID	FBrf0167658
Type of publication	Research paper
Offprint Available	No
External Crossreferences
PubMed ID	14704184
PubMed Abstract	Heterochromatin is a major component of higher eukaryotic genomes, but progress in understanding the molecular structure and composition of heterochromatin has lagged behind the production of relatively complete euchromatic genome sequences. The introduction of single-copy molecular-genetic entry points can greatly facilitate structure and sequence analysis of heterochromatic regions that are rich in repeated DNA. In this study, we report the isolation of 502 new P-element insertions into Drosophila melanogaster centric heterochromatin, generated in nine different genetic screens that relied on mosaic silencing (position-effect variegation, or PEV) of the yellow gene present in the transposon. The highest frequencies of recovery of variegating insertions were observed when centric insertions were used as the source for mobilization. We propose that the increased recovery of variegating insertions from heterochromatic starting sites may result from the physical proximity of different heterochromatic regions in germline nuclei or from the association of mobilizing elements with heterochromatin proteins. High frequencies of variegating insertions were also recovered when a potent suppressor of PEV (an extra Y chromosome) was present in both the mobilization and selection generations, presumably due to the effects of chromatin structure on P-element mobilization, insertion, and phenotypic selection. Finally, fewer variegating insertions were recovered after mobilization in females, in comparison to males, which may reflect differences in heterochromatin structure in the female and male germlines. FISH localization of a subset of the insertions confirmed that 98% of the variegating lines contain heterochromatic insertions and that these schemes produce a broader distribution of insertion sites. The results of these schemes have identified the most efficient methods for generating centric heterochromatin P insertions. In addition, the large collection of insertions produced by these screens provides molecular-genetic entry points for mapping, sequencing, and functional analysis of Drosophila heterochromatin.
Biosis
Zoological record
Associated Information
Comments
Text of personal communication
Associated files
Related Publications

Also Published As

Other Reference Information
Secondary IDs
Language of publication	English
Additional language(s) of abstract
ISBN
Place of publication
Published In
Abbreviation	Genetics
Title	Genetics
Authors
Volume range	1-
Year range	1916-
Page range
Publisher
Place of publication	Austin, TX, etc
Language of publication	English
ISBN/ISSN	0016-6731
CODEN	GENTAE
Data from Reference
Aberrations (3)
	C(1)RM C(1;1;Y)4 In(1)EN
Alleles (1)
	y¹
Constructs (1)
	P{SUPor-P}
Genes (1)
	y
Insertions (6)
	P{SUPor-P}B296 P{SUPor-P}B783.2 P{SUPor-P}B840.1 P{SUPor-P}C151 P{SUPor-P}J632.2 P{SUPor-P}K013.1