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Wei, H.C., Shu, H., Price, J.V. (2003). Functional genomic analysis of the 61D-61F region of the third chromosome of Drosophila melanogaster.  Genome 46(6): 1049--1058.
FlyBase ID
FBrf0167686
Publication Type
Research paper
Abstract

Assigning functional significance to completed genome sequences is one of the next challenges in biological science. Conventional genetic tools such as deficiency chromosomes help assign essential complementation groups to their corresponding genes. We describe an F2 genetic screen to identify lethal mutations within cytogenetic region 61D-61F of the third chromosome of Drosophila melanogaster. One hundred sixteen mutations were identified by their failure to complement both Df(3L)bab-PG and Df(3L)3C7. These alleles were assigned to 14 complementation groups and 9 deficiency intervals. Complementation groups were ordered using existing deficiencies, as well as new deficiencies generated in this study. With the aid of the genomic sequence, genetic and physical maps in the region were correlated by use of PCR to localize the breakpoints of deficiencies within a 268-kb genomic contig (GenBank accession No. AC005847). Six essential complementation groups were assigned to specific genes, including genes encoding a porphobilinogen deaminase and a Sac1-like protein.

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    Language of Publication
    English
    Additional Languages of Abstract
    French
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genome
    Title
    Genome
    Publication Year
    1987-
    ISBN/ISSN
    0831-2796
    Data From Reference