Reference Report

Reference
Citation	McGovern, V.L., Pacak, C.A., Sewell, S.T., Turski, M.L., Seeger, M.A. (2003). A targeted gain of function screen in the embryonic CNS of Drosophila. Mech. Dev. 120(10): 1193--1207. (Export to RIS)
FlyBase ID	FBrf0167840
Publication Type	Research paper
PubMed ID	14568107
PubMed Abstract	In order to identify genes involved in the development of the central nervous system (CNS) we have undertaken a gain of function screen in the embryonic CNS of Drosophila. Transposable P-elements and the UAS/GAL4 system were used to initiate transcription of genes in a pan-neural pattern using scaGAL4. Over 4100 individual P-element insertion lines were screened with monoclonal antibodies BP102 and 1D4 to visualize axon pathways. Twenty-five P-element insertions corresponding to 18 genes resulted in aberrant CNS axon pathfinding when misexpressed with scaGAL4. Genes involved in axon guidance, embryonic patterning, and cell cycle regulation were isolated. In addition, we identified several zinc finger transcription factors not previously implicated in axon guidance or CNS development. This group includes Squeeze, Kruppel homolog-1, Hepatocyte nuclear factor 4, and two uncharacterized genes, CG11966 and CG9650. Calnexin99A, a putative molecular chaperone, was isolated as well.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Aberrations (1)
	In(2LR)O
Alleles (33)
	CG9650^EP1617 CG11966^GS-A304 Cnx99A^EP3522 CycE^GS-A095 CycE^GS-A117 CycE^GS-M986 dpp^EP2232 drl^EP2076 Egfr^EPM865 EP-M269^EP-M269 GS(2)A096^GS-A096 hh^EP3521 hkb^GS-M962 hkb^GS-M1423b Kr-h1¹⁰⁶⁴² Kr-h1^EP2289 Kr-h1^GS-A029 Kr-h1^k04411 lea^EP2582 rho^EP3704 RhoGEF64C^EP3035 RhoGEF64C^EP3322 salm^GS-A265c Scer\GAL4^elav-C155 Scer\GAL4^GMR.PF Scer\GAL4^sca-4512 Scer\GAL4^sim.PS sqz⁰²¹⁰² sqz^GS-M1168 stg^EP3426 stg^EP3432 stg^GS-M1565 w¹¹¹⁸
Balancers (1)
	CyO-GS046
Constructs (2)
	P{GAL4-ninaE.GMR} P{GAL4-sim.S}
Genes (30)
	CG9650 CG11966 Cnx99A CycE dpp drl Egfr en EP-M269 eve Fas3 GS(2)A096 hh hkb Hnf4 Kr-h1 lea NetA NetB repo rho RhoGEF64C robo salm Scer\GAL4 sim sqz stg w wrapper
Insertions (34)
	P{EP}CG9650^EP1617 P{EP}Cnx99A^EP3522 P{EP}dpp^EP2232 P{EP}drl^EP2076 P{EP}Egfr^EPM865 P{EP}EP-M269^EP-M269 P{EP}hh^EP3521 P{EP}Kr-h1^EP2289 P{EP}Kr-h1^GS-A029 P{EP}l(1)G0007^EP764 P{EP}l(1)G0007^EP784 P{EP}lea^EP2582 P{EP}rho^EP3704 P{EP}RhoGEF64C^EP3035 P{EP}RhoGEF64C^EP3322 P{EP}rut^EP399 P{EP}stg^EP3426 P{EP}stg^EP3432 P{GAL4}sca⁴⁵¹² P{GawB}elav^C155 P{GSV1}046 P{GSV1}CG11966^GS-A304 P{GSV1}CycE^GS-A095 P{GSV1}CycE^GS-A117 P{GSV1}CycE^GS-M986 P{GSV1}GS(2)A096^GS-A096 P{GSV1}hkb^GS-M962 P{GSV1}hkb^GS-M1423b P{GSV1}salm^GS-A265c P{GSV1}sqz^GS-M1168 P{GSV1}stg^GS-M1565 P{lacW}Kr-h1^k04411 P{PZ}Kr-h1¹⁰⁶⁴² P{PZ}sqz⁰²¹⁰²