Reference Report

Reference
Citation	Zikova, M., Da Ponte, J.P., Dastugue, B., Jagla, K. (2003). Patterning of the cardiac outflow region in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 100(21): 12189--12194. (Export to RIS)
FlyBase ID	FBrf0167997
Publication Type	Research paper
PubMed ID	14519845
PubMed Abstract	Specification of bilateral cardiac primordia and formation of the linear heart tube are highly conserved from Drosophila to humans. However, subsequent heart morphogenesis involving nonmesodermal neural crest cells was thought to be specific for vertebrates. Here, we provide evidence that a group of nonmesodermal cells that we have named heart-anchoring cells (HANCs) contribute to heart morphogenesis in Drosophila. We show that the homeobox genes ladybird (lb) known to be involved in diversification of cardiac precursors are expressed in HANCs and required for their specification. Interestingly, the HANCs selectively contact the anterior cardiac cells, which express lb as well. Direct interaction between HANCs and cardiac cells is assisted by a pair of cardiac outflow muscles (COMs), each of which selectively attaches to both the lb-expressing cardiac cells and HANCs. COM muscles seem to ensure ventral bending of the heart tip and together with HANCs determine the spatial positioning of the cardiac outflow region. Experimentally depleted cardiac lb expression leads to the disruption of the contact between the tip of the heart and either the COM muscles or the HANC cells, indicating a pivotal morphogenetic role for the lb expression within the heart.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Aberrations (1)
	Df(3R)lb-Z
Alleles (11)
	bap²⁰⁸ C15^{dsRNA.Scer\UAS} Ecol\lacZ^Cyt-b5-01857 Ecol\lacZ^esg-4B7 Ecol\lacZ^how-B1-93F eve^Scer\UAS.cBa lbe^Scer\UAS.cJa lbl^Scer\UAS.cJa Scer\GAL4^arm.PS Scer\GAL4^how-24B Scer\GAL4^tin.cBa
Constructs (6)
	P{GAL4-arm.S} P{tin-Gal4.B} P{UAS.C15.dsRNA} P{UAS-eve.B} P{UAS-lbe.J} P{UAS-lbl.J}
Genes (13)
	bap C15 Ecol\lacZ esg eve lbe lbl Mhc Scer\GAL4 sr srp tin βTub60D
Insertions (4)
	P{GawB}how^24B P{lacW}B1-93F P{lacW}esg^4B7 P{PZ}Cyt-b5⁰¹⁸⁵⁷