|Citation||Strutt, D. (2003.10.8). Arm-Fz-GFP and Act5C-FRT-Stop-FRT-Fz-EYFP constructs. (Export to RIS)|
|Publication Type||Personal communication to FlyBase|
|Text of Personal Communication||
Date: Wed, 8 Oct 2003 11:05:24 \+0100 (BST)
From: David Strutt <D.Strutt@XXXXXXXXXXXXXXX>
To: Chihiro Yamada <cy200@XXXXXXXXXXXXXXX>
Subject: Re: FlyBase Query (cy2078)
Below, I've tried to compile a definitive list of the constructs actually
made and used in the Mol Cell paper, and then I've listed where the
Arm-Fz-GFP and Act5C-FRT-Stop-FRT-Fz-EYFP constructs have been
subsequently used, both in our publications and others. Hopefully this is
a complete list, although I might have missed a usage by another group.
\* Constructs made and used in experiments described in Strutt, 2001,
Molec. Cell 7(2): 367--375
Stable line with the FRT-Stop-FRT excised was generated for:
Act5C-FRT-Stop-FRT-FzP278L-EYFP = Act5C-FzP278L-EYFP
(Note that I didn't generate a stable line of the form Act5C-Fz-EYFP \-
expression of Fz-EYFP was always induced by co-expression of FLP in a
For simplicity, both EGFP and EYFP are referred to as GFP in the
manuscript. In the wing, both the Act5C and Arm promoter constructs give
the same results, so I also don't distinguish between them in the text.
\* Fz-GFP constructs used in subsequent publications
Strutt et al., 2002, Curr. Biol. 12(10): 813--824
In this paper I used both Arm-Fz-EGFP and Act5C-FRT-Stop-FRT-Fz-EYFP
transgenes. Arm-Fz-GFP is referred to as Fz-GFP in text and figures,
whereas Act-FRT-Stop-FRT-Fz-EYFP is referred to as Fz-YFP (and is only
used for one mosaic experiment in figure 4).
Strutt and Strutt, 2002, Dev. Cell 3(6): 851--863
Here again, both Arm-Fz-EGFP and Act5C-FRT-Stop-FRT-Fz-EYFP transgenes are
used. In figure 1, where Fz-GFP distribution in dsh and ds clones in
analysed, I used Arm-Fz-GFP and referred to it as Fz-GFP. In the
experiments in figures 2 and 3, where we induce Fz-GFP expression using
hsFLP1, we use the original Act5C-FRT-Stop-FRT-Fz-EYFP transgene from the
Mol Cell paper (and often abbreviated it to >>Fz on the figure panels).
Strutt and Strutt, 2003, Current Biology 13(16):1451--1457
Here we used only Arm-Fz-EGFP.
\* Our Fz-GFP constructs used by other groups in subsequent publications
Both Arm-Fz-EGFP and Act5C-FRT-Stop-FRT-Fz-EYFP transgenes have been
distributed, depending upon what was requested and whether it seemed
likely they would want to induce mosaic expression. However, I believe
that all the published examples used Arm-Fz-EGFP.
To my knowledge these are:
Tree et al., 2002, Cell 109(3): 371--381
Ma et al., 2003, Nature 421(6922): 543--547
Yang et al., 2002, Cell 108(5): 675--688
He & Adler, 2002, BMC Dev Biol. 2002 May 3;2(1):7
Adler, 2002, Dev Cell. 2002 May;2(5):525-35
\- although Ma et al and Yang et al don't actually show any pictures of
Fz-GFP ('data not shown')
Dr. David Strutt
Centre for Developmental Genetics
Department of Biomedical Science
University of Sheffield
Tel.: \+44 114 22 22 372
Fax.: \+44 114 22 22 788
|Research paper||Asymmetric localization of frizzled and the establishment of cell polarity in the Drosophila wing.
Strutt, 2001, Mol. Cell 7(2): 367--375 [FBrf0134764]
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|Language of Publication||English|
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