Reference Report

Reference
Citation	Strutt, D. (2003.10.8). Arm-Fz-GFP and Act5C-FRT-Stop-FRT-Fz-EYFP constructs. (Export to RIS)
FlyBase ID	FBrf0173063
Publication Type	Personal communication to FlyBase
PubMed ID
PubMed Abstract
Text of Personal Communication	Date: Wed, 8 Oct 2003 11:05:24 \+0100 (BST) From: David Strutt <D.Strutt@XXXXXXXXXXXXXXX> To: Chihiro Yamada <cy200@XXXXXXXXXXXXXXX> Subject: Re: FlyBase Query (cy2078) Dear Chihiro, .. Below, I've tried to compile a definitive list of the constructs actually made and used in the Mol Cell paper, and then I've listed where the Arm-Fz-GFP and Act5C-FRT-Stop-FRT-Fz-EYFP constructs have been subsequently used, both in our publications and others. Hopefully this is a complete list, although I might have missed a usage by another group. .. Best wishes, David \* Constructs made and used in experiments described in Strutt, 2001, Molec. Cell 7(2): 367--375 Arm-Fz-EGFP Act5C-FRT-Stop-FRT-Fz-EYFP Act5C-FRT-Stop-FRT-Fz^P278L-EYFP Act5C-Fz^dIntra-EYFP UAS-Fz-EGFP UAS-Fz^P278L UAS-Fz^dIntra-EYFP Stable line with the FRT-Stop-FRT excised was generated for: Act5C-FRT-Stop-FRT-Fz^P278L-EYFP = Act5C-Fz^P278L-EYFP (Note that I didn't generate a stable line of the form Act5C-Fz-EYFP \- expression of Fz-EYFP was always induced by co-expression of FLP in a Act5C-FRT-Stop-FRT-Fz-EYFP background.) For simplicity, both EGFP and EYFP are referred to as GFP in the manuscript. In the wing, both the Act5C and Arm promoter constructs give the same results, so I also don't distinguish between them in the text. \* Fz-GFP constructs used in subsequent publications Strutt et al., 2002, Curr. Biol. 12(10): 813--824 In this paper I used both Arm-Fz-EGFP and Act5C-FRT-Stop-FRT-Fz-EYFP transgenes. Arm-Fz-GFP is referred to as Fz-GFP in text and figures, whereas Act-FRT-Stop-FRT-Fz-EYFP is referred to as Fz-YFP (and is only used for one mosaic experiment in figure 4). Strutt and Strutt, 2002, Dev. Cell 3(6): 851--863 Here again, both Arm-Fz-EGFP and Act5C-FRT-Stop-FRT-Fz-EYFP transgenes are used. In figure 1, where Fz-GFP distribution in dsh and ds clones in analysed, I used Arm-Fz-GFP and referred to it as Fz-GFP. In the experiments in figures 2 and 3, where we induce Fz-GFP expression using hsFLP1, we use the original Act5C-FRT-Stop-FRT-Fz-EYFP transgene from the Mol Cell paper (and often abbreviated it to >>Fz on the figure panels). Strutt and Strutt, 2003, Current Biology 13(16):1451--1457 Here we used only Arm-Fz-EGFP. \* Our Fz-GFP constructs used by other groups in subsequent publications Both Arm-Fz-EGFP and Act5C-FRT-Stop-FRT-Fz-EYFP transgenes have been distributed, depending upon what was requested and whether it seemed likely they would want to induce mosaic expression. However, I believe that all the published examples used Arm-Fz-EGFP. To my knowledge these are: Tree et al., 2002, Cell 109(3): 371--381 Ma et al., 2003, Nature 421(6922): 543--547 Yang et al., 2002, Cell 108(5): 675--688 He & Adler, 2002, BMC Dev Biol. 2002 May 3;2(1):7 Adler, 2002, Dev Cell. 2002 May;2(5):525-35 \- although Ma et al and Yang et al don't actually show any pictures of Fz-GFP ('data not shown') \----------------------------------------------------------------------------- Dr. David Strutt Centre for Developmental Genetics Department of Biomedical Science University of Sheffield Western Bank Sheffield S10 2TN England Tel.: \+44 114 22 22 372 Fax.: \+44 114 22 22 788 e-mail: d.strutt@XXXXXXXXXXXXXXX
DOI
Related Publication(s)
Research paper	Asymmetric localization of frizzled and the establishment of cell polarity in the Drosophila wing. Strutt, 2001, Mol. Cell 7(2): 367--375 [FBrf0134764]
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Language of Publication	English
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Data from Reference
Alleles (11)
	fz^{Act5C.Scer\FRT.T:Avic\GFP-EYFP} fz^{Act5C.T:Avic\GFP-EYFP} fz^{arm.T:Avic\GFP-EGFP} fz^{P278L.Act5C.T:Avic\GFP-EYFP} fz^{P278L.Scer\FRT.Act5C.T:Avic\GFP-EYFP} fz^{P278L.Scer\UAS} fz^{Scer\UAS.T:Avic\GFP-EGFP} fz^{ΔIntra.Act5C.T:Avic\GFP-EYFP} fz^{ΔIntra.Scer\UAS.T:Avic\GFP-EYFP} T:Avic\GFP^EGFP T:Avic\GFP^EYFP
Constructs (8)
	P{Act5C(FRT.polyA)fz.EYFP} P{Act5C(-FRT)fz.EYFP} P{Act5C-fz.P278L.EYFP} P{Act5C-fz.ΔIntra.EYFP} P{arm-fz.GFP} P{UAS-fz.GFP} P{UAS-fz.P278L} P{UAS-fz.ΔIntra.EYFP}
Genes (2)
	fz T:Avic\GFP