Reference Report

Reference
Citation	Sathyanarayanan, S., Zheng, X., Xiao, R., Sehgal, A. (2004). Posttranslational regulation of Drosophila PERIOD protein by protein phosphatase 2A. Cell 116(4): 603--615. (Export to RIS)
FlyBase ID	FBrf0174437
Publication Type	Research paper
PubMed ID	14980226
PubMed Abstract	The posttranscriptional mechanisms that control the cycling of circadian clock protein levels are not known. Here we demonstrate a role for protein phosphatase 2A (PP2A) in the cyclic expression of the PER protein. PP2A regulatory subunits TWS and WDB target PER and stabilize it in S2 cells. In adult fly heads, expression of tws cycles robustly under control of the circadian clock. Hypomorphic tws mutants show delayed accumulation of PER, while overexpression of tws in clock neurons produces shorter, weaker rhythms. Reduction of PP2A activity reduces PER expression in central clock neurons and results in long periods and arrhythmia. In addition, overexpression of the PP2A catalytic subunit results in loss of behavioral rhythms and constitutive nuclear expression of PER. PP2A also affects PER phosphorylation in vitro and in vivo. We propose that the posttranslational mechanisms that drive cycling of PER require the rhythmic expression of PP2A.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (28)
	Avic\GFP^{dsRNA.E.1-642} CG4733^{dsRNA.4681-5597} cyc⁰¹ dco^{dsRNA.481-1232} dco^g dco^L dco^S Ecol\lacZ^T:SV5\V5 Ecol\lacZ^tws-02414 mts^{dn181.Scer\UAS} mts^dsRNA.1-690 mts^Scer\UAS.cHa per^Act5C.PD PP2A-B'^{dsRNA.795-1482} Scer\GAL4¹¹¹⁸ Scer\GAL4^P2.4.Pdf Scer\GAL4^tim.PE Scer\GAL4^tim.Scer\UAS tim^Act5C.PD tws⁶⁰ tws^{dsRNA.1227-1998} tws^{P1.Act5C.T:SV5\V5,T:Zzzz\His6} tws^{P2.Act5C.T:SV5\V5,T:Zzzz\His6} tws^Scer\UAS.cGa wdb^{Act5C.T:SV5\V5,T:Zzzz\His6} wdb^{dsRNA.399-1145} wdb^EP3113 wdb^EP3559
Constructs (6)
	P{GAL4-tim.E} P{Pdf-GAL4.P2.4} P{UAS-mts.dn181} P{UAS-mts.H} P{UAS-tim-GAL4} P{UAS-tws.G}
Genes (21)
	Avic\GFP CG4733 cyc dco Ecol\lacZ Hsp70Aa Hsp70Ab Hsp70Ba Hsp70Bb Hsp70Bbb Hsp70Bc mts Pdf per PP2A-B' Scer\GAL4 T:SV5\V5 T:Zzzz\His6 tim tws wdb
Insertions (4)
	P{EP}raps^EP3113 P{EP}wdb^EP3559 P{GAL4}1118 P{PZ}tws⁰²⁴¹⁴