Reference Report

Reference
Citation	Chen, G.C., Gajowniczek, P., Settleman, J. (2004). Rho-LIM kinase signaling regulates ecdysone-induced gene expression and morphogenesis during Drosophila metamorphosis. Curr. Biol. 14(4): 309--313. (Export to RIS)
FlyBase ID	FBrf0174477
Publication Type	Research paper
PubMed ID	14972681
PubMed Abstract	The steroid hormone 20-hydroxyecdysone (ecdysone) is the key regulator of postembryonic developmental transitions in insects and controls metamorphosis by triggering the morphogenesis of adult tissues from larvae. The Rho GTPase, which mediates cell shape change and migration, is also an essential regulator of tissue morphogenesis during development. Rho activity can modulate gene expression, in part, by activating LIM kinase (LIMK) and consequently affecting actin-induced SRF transcriptional activity. We have established a link between Rho-LIMK-SRF signaling and the ecdysone-induced transcriptional response during Drosophila development. Specifically, we determined that the Rho GTPase, via LIMK, regulates the expression of several ecdysone-responsive genes, including those encoding the ecdysone receptor itself, a downstream transcription factor (Br-C), and Stubble, a transmembrane protease required for proper leg formation. Stubble and Br-C mutants exhibit strong genetic interactions with several Rho pathway components in the formation of adult structures, but not with Rac or Cdc42. In cultured SL2 cells, inhibition of Rho, F-actin assembly, or SRF blocks the transcriptional response to ecdysone. Together, these findings indicate a link between Rho-LIMK signaling and steroid hormone-induced gene expression in the context of metamorphosis and thereby establish a novel role for the Rho GTPase in development.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (27)
	br¹ bs² bs^dsRNA.cCa Cdc42³ Cdc42⁴ dia¹ dia^k07135 LIMK1^{D522A.Scer\UAS} LIMK1^Scer\UAS.cCa mbc^2.35 Pak^KR.Scer\UAS Pkn⁰⁶⁷³⁶ Rac1^J11 Rac2³¹⁴ Rho1^72O Rho1^k02107b Rho1^Scer\UAS.cMa RhoGAPp190^{Scer\UAS.T:Hsap\MYC} RhoGEF2⁰⁴²⁹¹ rok¹ Sb^63b Sb⁷⁰ Scer\GAL4^69B Scer\GAL4^en-e16E Scer\GAL4^hs.PB Scer\GAL4^T80 zip^Ebr
Constructs (7)
	P{GAL4-Hsp70.PB} P{UAS-LIMK1.C} P{UAS-LIMK1.D522A} P{UAS-myc-RhoGAPp190.B} P{UAS-Pak.KR} P{UAS-Rho1.L} P{UAS-Rho1.Sph}
Genes (19)
	br bs Cdc42 dia EcR LIMK1 mbc Pak Pkn Rac1 Rac2 Rho1 RhoGAPp190 RhoGEF2 rok Sb Scer\GAL4 T:Hsap\MYC zip
Insertions (3)
	P{en2.4-GAL4}e16E P{GawB}69B P{GawB}T80