Reference Report

Reference
Citation	Marenda, D.R., Zraly, C.B., Dingwall, A.K. (2004). The Drosophila Brahma (SWI/SNF) chromatin remodeling complex exhibits cell-type specific activation and repression functions. Dev. Biol. 267(2): 279--293. (Export to RIS)
FlyBase ID	FBrf0174517
Publication Type	Research paper
PubMed ID	15013794
PubMed Abstract	The Brahma (Brm) complex of Drosophila melanogaster is a SWI/SNF-related chromatin remodeling complex required to correctly maintain proper states of gene expression through ATP-dependent effects on chromatin structure. The SWI/SNF complexes are comprised of 8-11 stable components, even though the SWI2/SNF2 (BRM, BRG1, hBRM) ATPase subunit alone is partially sufficient to carry out chromatin remodeling in vitro. The remaining subunits are required for stable complex assembly and/or proper promoter targeting in vivo. Our data reveals that SNR1 (SNF5-Related-1), a highly conserved subunit of the Brm complex, is required to restrict complex activity during the development of wing vein and intervein cells, illustrating a functional requirement for SNR1 in modifying whole complex activation functions. Specifically, we found that snr1 and brm exhibited opposite mutant phenotypes in the wing and differential misregulation of genes required for vein and intervein cell development, including rhomboid, decapentaplegic, thick veins, and blistered, suggesting possible regulatory targets for the Brm complex in vivo. Our genetic results suggest a novel mechanism for SWI/SNF-mediated gene repression that relies on the function of a 'core' subunit to block or shield BRM (SWI2/SNF2) activity in specific cells. The SNR1-mediated repression is dependent on cooperation with histone deacetylases (HDAC) and physical associations with NET, a localized vein repressor.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (8)
	Df(1)C246 Df(2L)net62 Df(2R)Px2 Df(3L)iro-2 Df(3L)ZN47 Df(3R)6-7 Df(3R)sbd105 In(3R)P(Pc³)
Alleles (83)
	ab¹ ash1⁴ ash1¹⁷ ash1²² Asx¹ brm² brm^{K804R.Scer\UAS.T:Ivir\HA1} brm^{K804R.T:Ivir\HA1} brm^unspecified bs² bs¹³⁴⁸ bs⁰³²⁶⁷ bs^k07909 corto^07128b corto^l1 corto^tsT18 cv¹ Dl^6B Dl^9P Dll⁵ Dll⁹ dpp^d-ho dpp^s1 E(spl)m8-HLH¹ E(z)⁶⁰ Ecol\lacZ^bs-03267 Ecol\lacZ^dpp.BS3.0 Ecol\lacZ^rho-X81 Ecol\lacZ^tkv-k16713 Egfr^E1 en⁴ ex⁶⁹⁷ gpp^61A gpp^94A gpp^XXV gro¹ gro^C105 H² Hdac3^B Hsc70-4⁰³⁵⁵⁰ Hsc70-4^L3929 kis¹ kis^k13416 kni^ri-1 lawc^EF250 lawc^p1 Me¹ mor¹ N^Ax-tsl net¹ osa² osa⁰⁰⁰⁹⁰ Pc¹ Pc⁴ Pcl¹¹ Psc¹ Psc^e22 px⁷² rho^ve-1 Rpd3⁰⁴⁵⁵⁶ RpII215¹ RpII215³ RpII215⁸ S¹ sbb⁰⁴⁴⁴⁰ Sce^D1 Scer\GAL4^69B Scm^D1 Scr⁷ Scr¹⁷ sd¹ sens^Ly-1 Snr1^E1 Snr1^R3 Su(z)2^1.a1 Taf1¹ Taf1^EP421 Taf1^XS-2232 tkv¹ tkv^k16713 trx¹ trx^E2 vn^C221
Constructs (2)
	P{BS3.0} P{UAS-brm.K804R.T:HA1}
Genes (51)
	ab ash1 Asx brm bs corto cv Dl Dll dpp E(spl)m8-HLH E(z) Ecol\lacZ Egfr en ex gpp gro H Hdac3 Hsc70-4 kis kni lawc Me mor N net osa Pc Pcl Psc px rho Rpd3 RpII215 S sbb Sce Scer\GAL4 Scm Scr sd sens Snr1 Su(z)2 T:Ivir\HA1 Taf1 tkv trx vn
Insertions (4)
	P{GawB}69B P{lacW}tkv^k16713 P{lwB}X81 P{PZ}bs⁰³²⁶⁷