Reference Report

Reference
Citation	Shi, W.Y., Skeath, J.B. (2004). The Drosophila RCC1 homolog, Bj1, regulates nucleocytoplasmic transport and neural differentiation during Drosophila development. Dev. Biol. 270(1): 106--121. (Export to RIS)
FlyBase ID	FBrf0174533
Publication Type	Research paper
PubMed ID	15136144
PubMed Abstract	The Bj1 gene encodes the Drosophila homolog of RCC1, the guanine-nucleotide exchange factor for RanGTPase. Here, we provide the first phenotypic characterization of a RCC1 homolog in a developmental model system. We identified Bj1 (dRCC1) in a genetic screen to identify mutations that alter central nervous system development. We find that zygotic dRCC1 mutant embryos exhibit specific defects in the development and differentiation of lateral CNS neurons although cell division and the cell cycle appear grossly normal. dRCC1 mutant nerve cords contain abnormally large cells with compartmentalized nuclei and exhibit increased transcription in the lateral CNS. As RCC1 is an important component of the nucleocytoplasmic transport machinery, we find that dRCC1 function is required for nuclear import of nuclear localization signal sequence (NLS)-carrying cargo molecules. Finally, we show that dRCC1 is required for cell proliferation and/or survival during germline, eye and wing development and that dRCC1 appears to facilitate apoptosis.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (1)
	Df(3L)ZN47
Alleles (17)
	Ecol\lacZ^{Act5C.W.T:Btau\MAPT} Ecol\lacZ^{Scer\FRT.W.Act5C.T:Btau\MAPT} Ecol\lacZ^{Scer\UAS.T:SV40\nls2} l(3)CL-L¹ Rcc1⁸³⁷ Rcc1^{del.Scer\UAS.T:Hsap\MYC} Rcc1^EP3630 Rcc1^FF32 Rcc1^OreR Rcc1^Scer\UAS.cSa Rcc1^unspecified Rcc1^UU92 Rcc1^VV179 Rcc1^XX138 Scer\GAL4^ey.PH Scer\GAL4^sca-109-68 W^GMR.PG
Constructs (7)
	P{Act5C-FRT-Draf-FRT-tau-lacZ.W} P{Act5C-tau-lacZ.W} P{GAL4-ey.H} P{GMR-hid} P{UAS-Bj1.del.MYC} P{UAS-Bj1.S} P{UAS-lacZ.NZ}
Genes (18)
	CycA Ecol\lacZ en eve Hel25E His3 l(3)CL-L Lam Ran Rcc1 Scer\GAL4 snRNP-U1-70K stg T:Btau\MAPT T:Hsap\MYC T:SV40\nls2 W zfh1
Insertions (3)
	P{EP}Rcc1^EP3630 P{GawB}sca^109-68 P{GMR-hid}SS3