Reference Report

Reference
Citation	Santos, A.C., Lehmann, R. (2004). Isoprenoids control germ cell migration downstream of HMGCoA reductase. Dev. Cell 6(2): 283--293. (Export to RIS)
FlyBase ID	FBrf0174539
Publication Type	Research paper
PubMed ID	14960281
PubMed Abstract	3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCoAr) provides attractive cues to Drosophila germ cells, guiding them toward the embryonic gonad. However, it remains unclear how HMGCoAr mediates this attraction. In a genomic analysis of the HMGCoAr pathway, we found that the fly genome lacks several enzymes required for cholesterol biosynthesis, ruling out cholesterol and cholesterol-derived proteins as mediators of PGC migration. Genetic analysis of the pathway revealed that two enzymes, farnesyl-diphosphate synthase and geranylgeranyl-diphosphate synthase, required for the production of isoprenoids, act downstream of HMGCoAr in germ cell migration. Consistent with a role in geranylgeranylation, embryos deficient in geranylgeranyl transferase type I show germ cell migration defects. Our data, together with similar findings in zebrafish, implicate an isoprenylated protein in germ cell attraction. The specificity and evolutionary conservation of the HMGCoAr pathway for germ cells suggest that an attractant common to invertebrates and vertebrates guides germ cells in early embryos.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Alleles (11)
	Fpps^k06103 Fpps^Scer\UAS.cSa Hmgcr⁰¹¹⁵² Hmgcr^clb1 Hmgcr^{Scer\UAS.cvDa} Hmgcr^unspecified qm^L14.4 qm^Scer\UAS.cSa Scer\GAL4^elav-C155 βggt-I^S-483 βggt-I^S-2554
Constructs (3)
	P{UAS-Fpps.S} P{UAS-Hmgcr.vD} P{UAS-qm.S}
Genes (18)
	CG1998 CG2976 CG5919 CG8239 CG10268 CG11162 CG12007 CG17565 CG33671 CG33672 Cyp4d1 Fpps Hmgcr qm Scer\GAL4 vas βggt-I βggt-II
Insertions (2)
	P{GawB}elav^C155 P{lacW}Fpps^k06103