Reference Report

Reference
Citation	Kimura, K., Kodama, A., Hayasaka, Y., Ohta, T. (2004). Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogaster. Development 131(7): 1597--1606. (Export to RIS)
FlyBase ID	FBrf0174562
Publication Type	Research paper
PubMed ID	14998927
PubMed Abstract	At the last step of metamorphosis in Drosophila, the wing epidermal cells are removed by programmed cell death during the wing spreading behavior after eclosion. The cell death was accompanied by DNA fragmentation demonstrated by the TUNEL assay. Transmission electron microscopy revealed that this cell death exhibited extensive vacuoles, indicative of autophagy. Ectopic expression of an anti-apoptotic gene, p35, inhibited the cell death, indicating the involvement of caspases. Neck ligation and hemolymph injection experiments demonstrated that the cell death is triggered by a hormonal factor secreted just after eclosion. The timing of the hormonal release implies that the hormone to trigger the death might be the insect tanning hormone, bursicon. This was supported by evidence that wing cell death was inhibited by a mutation of rickets, which encodes a G-protein coupled receptor in the glycoprotein hormone family that is a putative bursicon receptor. Furthermore, stimulation of components downstream of bursicon, such as a membrane permeant analog of cAMP, or ectopic expression of constitutively active forms of G proteins or PKA, induced precocious death. Conversely, cell death was inhibited in wing clones lacking G protein or PKA function. Thus, activation of the cAMP/PKA signaling pathway is required for transduction of the hormonal signal that induces wing epidermal cell death after eclosion.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (12)
	Avic\GFP^{Scer\UAS.T:SV40\nls2} BacA\p35^Scer\UAS.cHa Gαs^{Q215L.Scer\UAS} Gαs^R60 Gαs^Scer\UAS.cCa His2Av^{T:Avic\GFP-S65T} Mmus\Pkaca^mC.Scer\UAS Pka-C1^E95 Pka-R1^BDK.Scer\UAS rk¹ Scer\GAL4^en-e16E T:Avic\GFP^S65T
Constructs (7)
	P{His2Av^{T:Avic\GFP-S65T}} P{UAS-GFP.nls} P{UAS-G-sα60A.C} P{UAS-G-sα60A.Q215L} P{UAS-p35.H} P{UAS-PKA.mC} P{UAS-Pka-R1.BDK}
Genes (11)
	Avic\GFP BacA\p35 Gαs His2Av Mmus\Pkaca Pka-C1 Pka-R1 rk Scer\GAL4 T:Avic\GFP T:SV40\nls2
Insertions (1)
	P{en2.4-GAL4}e16E