Reference Report

Reference
Citation	Strutt, H., Mundy, J., Hofstra, K., Strutt, D. (2004). Cleavage and secretion is not required for Four-jointed function in Drosophila patterning. Development 131(4): 881--890. (Export to RIS)
FlyBase ID	FBrf0174590
Publication Type	Research paper
PubMed ID	14757640
PubMed Abstract	Four-jointed (fj) is required for proximodistal growth and planar polarity in Drosophila tissues. It encodes a predicted type II transmembrane protein with putative signal peptidase sites in its transmembrane domain, and its C terminus is secreted. Fj has therefore been proposed to act as a secreted signalling molecule. We show that Fj protein has a graded distribution in eye and wing imaginal discs, and is largely localised to the Golgi in vivo and in transfected cells. Forms of Fj that are constitutively secreted or anchored in the Golgi were assayed for function in vivo. We find that cleavage and secretion of Fj is not necessary for activity, and that Golgi-anchored Fj has increased activity over wild type. fj has similar phenotypes to those caused by mutations in the cadherin-encoding genes fat (ft) and dachsous (ds). We show that fj interacts genetically with ft and ds in planar polarity and proximodistal patterning. We propose that Fj may act in the Golgi to regulate the activity of Ft and Ds.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (30)
	ds¹ ds^38k ds^UAO71 Ecol\lacZ^arm.PV Ecol\lacZ^fj.t14 fj^{102-583.MtnA.T:Rnor\CD2} fj^{102-583.Scer\UAS.T:Rnor\CD2} fj^{109-583.MtnA.T:Hsap\GALNT3,T:Zzzz\FLAG} fj^{109-583.MtnA.T:Hsap\GALNT3} fj^{109-583.Scer\UAS.T:Hsap\GALNT3,T:Zzzz\FLAG} fj^{109-583.Scer\UAS.T:Hsap\GALNT3} fj^+t14 fj^+t24 fj^d1 fj^Scer\UAS.cZa fj^{t14.T:Hsap\GALNT3} fj^{t14.T:Rnor\CD2} fj^{Un.MtnA.T:Zzzz\FLAG} fj^Un.MtnA fj^{Un.Scer\UAS.T:Zzzz\FLAG} fj^Un.Scer\UAS fj^Un.t14 fj^unspecified ft¹ ft⁴ ft^G-rv Mmus\Fjx1^{36-437.Scer\UAS.T:Hsap\GALNT3} Scer\GAL4^Act5C.PI Scer\GAL4^bi-omb-Gal4 Scer\GAL4^{Scer\FRT.Act5C}
Constructs (18)
	P{Act5C-GAL4} P{Act5C-GAL4-w} P{arm-lacZ.V} P{AyGAL4} P{AyGAL4-w} P{fj.14-lacZ} P{fj.+t14} P{fj.+t24} P{fj.t14::CD2} P{fj.t14::GNT} P{fj.Un.t14} P{UAS-fj::CD2} P{UAS-fj.109-583::GNT} P{UAS-fj.109-583.GNT.FLAG} P{UAS-fj.Un::FLAG} P{UAS-fj.Un} P{UAS-fj} P{UAS-fjx1.36-437.GNT}
Genes (11)
	ci ds Ecol\lacZ fj ft Hsap\GALNT3 Mmus\Fjx1 Scer\GAL4 T:Hsap\GALNT3 T:Rnor\CD2 T:Zzzz\FLAG
Insertions (1)
	P{GAL4}bi^omb-Gal4