Reference Report

Reference
Citation	Joshi, R., Venkatesh, K., Srinivas, R., Nair, S., Hasan, G. (2004). Genetic dissection of itpr gene function reveals a vital requirement in aminergic cells of Drosophila larvae. Genetics 166(1): 225--236. (Export to RIS)
FlyBase ID	FBrf0174703
Publication Type	Research paper
PubMed ID	15020420
PubMed Abstract	Signaling by the second messenger inositol 1,4,5-trisphosphate is thought to affect several developmental and physiological processes. Mutants in the inositol 1,4,5-trisphosphate receptor (itpr) gene of Drosophila exhibit delays in molting while stronger alleles are also larval lethal. In a freshly generated set of EMS alleles for the itpr locus we have sequenced and identified single point mutations in seven mutant chromosomes. The predicted allelic strength of these mutants matches the observed levels of lethality. They range from weak hypomorphs to complete nulls. Interestingly, lethality in three heteroallelic combinations has a component of cold sensitivity. The temporal focus of cold sensitivity lies in the larval stages, predominantly at second instar. Coupled with our earlier observation that an itpr homozygous null allele dies at the second instar stage, it appears that there is a critical period for itpr gene function in second instar larvae. Here we show that the focus of this critical function lies in aminergic cells by rescue with UAS-itpr and DdCGAL4. However, this function does not require synaptic activity, suggesting that InsP(3)-mediated Ca(2+) release regulates the neurohormonal action of serotonin.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (29)
	Ctet\TeTxLC^TNT.Scer\UAS Itp-r83A^90B.0 Itp-r83A⁰⁵⁶¹⁶ Itp-r83A^B4 Itp-r83A^hc1 Itp-r83A^hc7 Itp-r83A^ka901 Itp-r83A^ka904 Itp-r83A^ka1091 Itp-r83A^kv862 Itp-r83A^sa54 Itp-r83A^Scer\UAS.cVa Itp-r83A^sv35 Itp-r83A^sv273 Itp-r83A^sv520 Itp-r83A^ug3 Itp-r83A^ug4 Itp-r83A^wc361 Itp-r83A^wc703 Itp-r83A^XR12 Scer\GAL4^12.1 Scer\GAL4^arm.PS Scer\GAL4^Cha.7.4 Scer\GAL4^Ddc.PL Scer\GAL4^dimm-929 Scer\GAL4^elav-C155 Scer\GAL4^{neur-GAL4-A101} Scer\GAL4^pros.PMG W^Scer\UAS.cZa
Constructs (7)
	P{Cha-GAL4.7.4} P{Ddc-GAL4.L} P{GAL4-arm.S} P{GAL4-pros.MG} P{UAS-Itp-r83A.V} P{UAS-TeTxLC.tnt} P{UAS-W.Z}
Genes (5)
	Ctet\TeTxLC Ddc Itp-r83A Scer\GAL4 W
Insertions (5)
	P{GAL4}12.1 P{GawB}dimm⁹²⁹ P{GawB}elav^C155 P{GawB}neur^GAL4-A101 P{PZ}Itp-r83A⁰⁵⁶¹⁶