Reference Report

Reference
Citation	Myster, S.H., Wang, F., Cavallo, R., Christian, W., Bhotika, S., Anderson, C.T., Peifer, M. (2004). Genetic and bioinformatic analysis of 41C and the 2R heterochromatin of Drosophila melanogaster: a window on the heterochromatin-euchromatin junction. Genetics 166(2): 807--822. (Export to RIS)
FlyBase ID	FBrf0174713
Publication Type	Research paper
PubMed ID	15020470
PubMed Abstract	Genomic sequences provide powerful new tools in genetic analysis, making it possible to combine classical genetics with genomics to characterize the genes in a particular chromosome region. These approaches have been applied successfully to the euchromatin, but analysis of the heterochromatin has lagged somewhat behind. We describe a combined genetic and bioinformatics approach to the base of the right arm of the Drosophila melanogaster second chromosome, at the boundary between pericentric heterochromatin and euchromatin. We used resources provided by the genome project to derive a physical map of the region, examine gene density, and estimate the number of potential genes. We also carried out a large-scale genetic screen for lethal mutations in the region. We identified new alleles of the known essential genes and also identified mutations in 21 novel loci. Fourteen complementation groups map proximal to the assembled sequence. We used PCR to map the endpoints of several deficiencies and used the same set of deficiencies to order the essential genes, correlating the genetic and physical map. This allowed us to assign two of the complementation groups to particular "computed/curated genes" (CGs), one of which is Nipped-A, which our evidence suggests encodes Drosophila Tra1/TRRAP.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (26)
	Df(2R)244 Df(2R)247 Df(2R)Dark2 Df(2R)M41A2 Df(2R)M41A4 Df(2R)M41A8 Df(2R)M41A10 Df(2R)nap1 Df(2R)NC13 Df(2R)NC32 Df(2R)NC50 Df(2R)NC68 Df(2R)NC73 Df(2R)NC98 Df(2R)NC125 Df(2R)NC167 Df(2R)NC168 Df(2R)NC191 Df(2R)NC195 Df(2R)NC202 Df(2R)NC207 Df(2R)NC218 Df(2R)NC219 Df(2R)NC228 Df(2R)NC1198 Df(2R)Nipped-D
Alleles (12)
	CG17683^NC109 IntS3^IR23 IntS3^NC89 l(2)NC136^KG10496 Nipped-A^357.2 Nipped-A^NC116 Nipped-A^NC186 Nipped-B^NC138 scaf^KG05129 uex^IR3 uex^NC9 vlc^EY01995
Genes (65)
	ap Atf6 BubR1 CG1294 CG1344 CG3107 CG8245 CG10395 CG10396 CG10417 CG10465 CG11665 CG12547 CG14464 CG17082 CG17337 CG17478 CG17479 CG17486 CG17508 CG17528 CG17683 CG17883 CG30438 CG30440 CG30441 CG30442 CG40127 CG40279 CG40290 CR41440 d4 Fis1 gus IntS3 kune l(2)41Ae l(2)309 l(2)NC12 l(2)NC14 l(2)NC30 l(2)NC45 l(2)NC47 l(2)NC83 l(2)NC85 l(2)NC114 l(2)NC133 l(2)NC136 l(2)NC139 l(2)NC148 l(2)NC192 l(2)NC204 laccase2 Nipped-A Nipped-B p120ctn rl RpL38 scaf Stlk sxc TpnC4 TpnC41C uex vlc
Insertions (14)
	P{EPgy2}EY07370 P{EPgy2}vlc^EY01995 P{SUPor-P}CG30438^KG02589 P{SUPor-P}CG30438^KG06088 P{SUPor-P}CR40282^KG08252 P{SUPor-P}d4^KG01463 P{SUPor-P}KG00632 P{SUPor-P}KG01086 P{SUPor-P}KG02079 P{SUPor-P}KG03359 P{SUPor-P}KG05308 P{SUPor-P}l(2)NC136^KG10496 P{SUPor-P}Nipped-A^KG10162 P{SUPor-P}scaf^KG05129
Natural transposons (2)
	1360 INE-1