Reference Report

Reference
Citation	Sun, X.X., Hodge, J.J., Zhou, Y., Nguyen, M., Griffith, L.C. (2004). The eag potassium channel binds and locally activates calcium/calmodulin-dependent protein kinase II. J. Biol. Chem. 279(11): 10206--10214. (Export to RIS)
FlyBase ID	FBrf0174770
Publication Type	Research paper
PubMed ID	14699099
PubMed Abstract	Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) has been implicated in the regulation of neuronal excitability in many systems. Recent studies suggest that local regulation of membrane potential can have important computational consequences for neuronal function. In Drosophila, CaMKII regulates the eag potassium channel, but if and how this regulation was spatially restricted was unknown. Using coimmunoprecipitation from head extracts and in vitro binding assays, we show that CaMKII and Eag form a stable complex and that association with Eag activates CaMKII independently of CaM and autophosphorylation. Ca(2+)/CaM is necessary to initiate binding of CaMKII to Eag but not to sustain association because binding persists when CaM is removed. The Eag CaMKII-binding domain has homology to the CaMKII autoregulatory region, and the constitutively active CaMKII mutant, T287D, binds Eag Ca(2+)-independently in vitro and in vivo. These results favor a model in which the CaMKII-binding domain of Eag displaces the CaMKII autoinhibitory region. Displacement results in autophosphorylation-independent activation of CaMKII which persists even when Ca(2+) levels have gone down. Activity-dependent binding to this potassium channel substrate allows CaMKII to remain locally active even when Ca(2+) levels have dropped, providing a novel mechanism by which CaMKII can regulate excitability locally over long time scales.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Biol. Chem.
Title	Journal of Biological Chemistry
Publication Year	1905-
ISBN/ISSN	0021-9258
Data from Reference
Alleles (3)
	eag^sc29 n-syb^{Scer\UAS.cEa.T:Avic\GFP} Scer\GAL4^elav-C155
Constructs (1)
	P{UAS-n-syb-GFP.E}
Genes (5)
	CaMKII eag n-syb Scer\GAL4 T:Avic\GFP
Insertions (1)
	P{GawB}elav^C155