Reference Report

Reference
Citation	Bachmann, A., Timmer, M., Sierralta, J., Pietrini, G., Gundelfinger, E.D., Knust, E., Thomas, U. (2004). Cell type-specific recruitment of Drosophila Lin-7 to distinct MAGUK-based protein complexes defines novel roles for Sdt and Dlg-S97. J. Cell Sci. 117(10): 1899--1909. (Export to RIS)
FlyBase ID	FBrf0174808
Publication Type	Research paper
PubMed ID	15039455
PubMed Abstract	Stardust (Sdt) and Discs-Large (Dlg) are membrane-associated guanylate kinases (MAGUKs) involved in the organization of supramolecular protein complexes at distinct epithelial membrane compartments in Drosophila. Loss of either Sdt or Dlg affects epithelial development with severe effects on apico-basal polarity. Moreover, Dlg is required for the structural and functional integrity of synaptic junctions. Recent biochemical and cell culture studies have revealed that various mammalian MAGUKs can interact with mLin-7/Veli/MALS, a small PDZ-domain protein. To substantiate these findings for their in vivo significance with regard to Sdt- and Dlg-based protein complexes, we analyzed the subcellular distribution of Drosophila Lin-7 (DLin-7) and performed genetic and biochemical assays to characterize its interaction with either of the two MAGUKs. In epithelia, Sdt mediates the recruitment of DLin-7 to the subapical region, while at larval neuromuscular junctions, a particular isoform of Dlg, Dlg-S97, is required for postsynaptic localization of DLin-7. Ectopic expression of Dlg-S97 in epithelia, however, was not sufficient to induce a redistribution of DLin-7. These results imply that the recruitment of DLin-7 to MAGUK-based protein complexes is defined by cell-type specific mechanisms and that DLin-7 acts downstream of Sdt in epithelia and downstream of Dlg at synapses.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Alleles (14)
	dlg1⁷ dlg1¹² dlg1¹⁴ dlg1^{S97N.Scer\UAS.T:Avic\GFP-EGFP} dlg1^{Scer\UAS.T:Avic\GFP} Scer\GAL4^C57 Scer\GAL4^C164 Scer\GAL4^da.G32 Scer\GAL4^ptc-559.1 sdt^{Scer\UAS.MAGUK} T:Avic\GFP^EGFP veli^{dsRNA.Scer\UAS} veli^{Scer\UAS.T:Zzzz\FLAG} w^+mC
Constructs (5)
	P{UAS-dlg1.S97N-EGFP} P{UAS-eGFP-dlg1-S48S} P{UAS-sdt.MAGUK} P{UAS-veli.dsRNA} P{UAS-veli.FLAG}
Genes (8)
	CASK dlg1 Scer\GAL4 sdt T:Avic\GFP T:Zzzz\FLAG veli w
Insertions (3)
	P{GawB}C57 P{GawB}C164 P{GawB}ptc^559.1