Reference Report

Reference
Citation	Bharathi, V., Pallavi, S.K., Bajpai, R., Emerald, B.S., Shashidhara, L.S. (2004). Genetic characterization of the Drosophila homologue of coronin. J. Cell Sci. 117(10): 1911--1922. (Export to RIS)
FlyBase ID	FBrf0174809
Publication Type	Research paper
PubMed ID	15090595
PubMed Abstract	We report cloning and characterization of coro, which codes for the Drosophila homologue of the F-actin binding protein coronin. Viable alleles of coro produce a variety of phenotypes in leg, wing and eye development, which are similar to the phenotypes observed as a result of mutations in genes associated with the actin cytoskeleton and/or membrane trafficking. Homozygous lethal mutations in coro results in the disruption of the actin cytoskeleton in wing imaginal discs. Formation of both basolateral septate junctions and apical adherens junctions are also adversely affected in epithelial cells. Both viable and lethal alleles of coro show genetic interactions with syntaxin1A, a gene required for membrane trafficking. They also show enhanced response to over-expression of Decapentaplegic (Dpp) and its receptor Thick vein. Tracing of Dpp morphogen using a Dpp::GFP fusion construct suggested defects in the endocytic pathway, which resulted in uniform distribution of Dpp along the AP axis rather than a gradient from the AP boundary. Our results provide a genetic link between endocytosis/exocytosis events involving F actin-coated vesicles and the establishment of morphogen gradient.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Alleles (19)
	coro⁴⁰⁹ coro^ex2 coro^ex3 coro^ex4 coro^ex6 coro^ex7 coro^ex8 coro^ex9 coro^ex10 coro^ex11 coro^ex12 dpp^{Scer\UAS.T.T:Avic\GFP-EGFP} Ecol\lacZ^bi-Pol-1 Ecol\lacZ^Scer\UAS.cUa Scer\GAL4^coro-409 Scer\GAL4^dpp.blk1 Syx1A^DN.Scer\UAS T:Avic\GFP^EGFP tkv^{Q253D.Scer\UAS.cNb}
Constructs (9)
	P{GAL4} P{GAL4-dpp.blk1} P{GAL4-Δex8} P{GAL4-Δex10} P{GAL4-Δex11} P{UAS-dpp.GFP.T} P{UAS-lacZ.U} P{UAS-Syx1A.DN} P{UAS-tkv.Q253D.Nb}
Genes (20)
	arm bi CG9447 ci coro ct Cyp6u1 Dll dpp Ecol\lacZ en Fas3 salm Scer\GAL4 SdhB Syx1A T:Avic\GFP tkv vimar wg
Insertions (5)
	P{GAL4}coro⁴⁰⁹ P{GAL4-Δex8}coro^ex8 P{GAL4-Δex10}coro^ex10 P{GAL4-Δex11}coro^ex11 P{lacW}bi^Pol-1