Home
Reference Report
| Reference | |||
|---|---|---|---|
| Citation | Zhou, X., Zhou, B., Truman, J.W., Riddiford, L.M. (2004). Overexpression of broad: a new insight into its role in the Drosophila prothoracic gland cells. J. Exp. Biol. 207(7): 1151--1161. (Export to RIS) | ||
| FlyBase ID | FBrf0174866 | ||
| Publication Type | Research paper | ||
| PubMed ID | 14978057 | ||
| PubMed Abstract | Insect molting is triggered by ecdysteroids, which are produced in the prothoracic glands (PG). The broad (br) gene is one of the 'early genes' directly regulated by ecdysteroids. Ectopic expression of the BR-Z3 isoform in early second instar Drosophila larvae (L2) before the rise of the ecdysteroid titer prevented molting to the third instar, but the larvae subsequently formed L2 prepupae after prolonged feeding. When these larvae were fed on diet containing 20-hydroxyecdysone (20E), they formed pharate third instar larvae. The critical weight for normal L3 pupariation of w(1118) larvae was found to be 0.8 mg and that for L2 pupariation was 0.45 mg. We also defined a threshold weight for metamorphosis of 0.3 mg, above which L2 larvae will metamorphose when provided with 20E. BR-Z3 apparently works through the PG cells of the ring gland but not the putative neurosecretory cells that drive ecdysone secretion, because ectopic expression of BR-Z3 specifically in the ring gland caused 53% of the larvae to become permanent first instar larvae. Driving other BR isoforms in the ring gland prevented larval molting or pupariation to varying degrees. These molting defects were rescued by feeding 20E. Overexpression of each of the BR isoforms caused degeneration of the PG cells but on different time courses, indicating that BR is a signal for the degeneration of the PG cells that normally occurs during the pupal-adult transition. | ||
| DOI | |||
| Related Publication(s) | |||
| Review | Fast developers. Phillips, 2004, J. Exp. Biol. 207(7): 1057 [FBrf0179384] |
||
Recent Updates
|
|||
| Description |
What does this section display?
This section contains items that were added to this record for each release.
It currently only tracks new links between this FlyBase report and other
FlyBase data classes (e.g. genes, references, stocks) or controlled
vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
|
||
| Update Feed |
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your
feed reader.
|
||
| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
|
Associated Information
|
|||
| Comments | |||
| Associated Files | |||
|
Other Information
|
|||
| Secondary IDs | |||
| Language of Publication | English | ||
| Additional Languages of Abstract | |||
| Also Published As | |||
|
Parent Publication
|
|||
| Publication Type | Journal | ||
| Abbreviation | J. Exp. Biol. | ||
| Title | Journal of Experimental Biology | ||
| Publication Year | 1930- | ||
| ISBN/ISSN | 0022-0949 | ||
|
Data from Reference
|
|||
| Alleles (13)
|
|||
| Constructs (9)
|
|||
| Genes (2)
|
|||
| Insertions (4)
|
|||