Reference Report

Reference
Citation	Marin, M.C., Rodriguez, J.R., Ferrus, A. (2004). Transcription of Drosophila Troponin I gene is regulated by two conserved, functionally identical, synergistic elements. Mol. Biol. Cell 15(3): 1185--1196. (Export to RIS)
FlyBase ID	FBrf0174929
Publication Type	Research paper
PubMed ID	14718563
PubMed Abstract	The Drosophila wings-up A gene encodes Troponin I. Two regions, located upstream of the transcription initiation site (upstream regulatory element) and in the first intron (intron regulatory element), regulate gene expression in specific developmental and muscle type domains. Based on LacZ reporter expression in transgenic lines, upstream regulatory element and intron regulatory element yield identical expression patterns. Both elements are required for full expression levels in vivo as indicated by quantitative reverse transcription-polymerase chain reaction assays. Three myocyte enhancer factor-2 binding sites have been functionally characterized in each regulatory element. Using exon specific probes, we show that transvection is based on transcriptional changes in the homologous chromosome and that Zeste and Suppressor of Zeste 3 gene products act as repressors for wings-up A. Critical regions for transvection and for Zeste effects are defined near the transcription initiation site. After in silico analysis in insects (Anopheles and Drosophila pseudoobscura) and vertebrates (Ratus and Coturnix), the regulatory organization of Drosophila seems to be conserved. Troponin I (TnI) is expressed before muscle progenitors begin to fuse, and sarcomere morphogenesis is affected by TnI depletion as Z discs fail to form, revealing a novel developmental role for the protein or its transcripts. Also, abnormal stoichiometry among TnI isoforms, rather than their absolute levels, seems to cause the functional muscle defects.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Aberrations (1)
	In(1)z^ae(bx)
Alleles (30)
	bin^R22 Ecol\lacZ^{2.6.wupA-Antp} Ecol\lacZ^{2.7.wupA-Antp} Ecol\lacZ^{4.7.wupA-Antp} Ecol\lacZ^4.wupA-Antp Ecol\lacZ^{IRE-0.3A.wupA-Antp} Ecol\lacZ^{IRE-0.3B.wupA-Antp} Ecol\lacZ^{IRE-0.3C.wupA-Antp} Ecol\lacZ^{IRE-0.89.wupA-Antp} Ecol\lacZ^{IRE-1.7.wupA-Antp} Ecol\lacZ^{IRE-1.8.wupA-Antp} Ecol\lacZ^{IRE-2.7.wupA-Antp} Ecol\lacZ^{IRE-2.9.wupA-Antp} Ecol\lacZ^{URE-0.5.wupA-Antp} Ecol\lacZ^{URE-2.0.wupA-Antp} gypsy\su(Hw)BR⁴³⁰ l(1)16Fe²³⁴³⁷ l(1)16Fm²³⁴³⁷ lmd^A388 Mef2^22-21 Mhc^D41 tin³⁴⁶ wupA^D3 wupA^hdp-2 wupA^hdp-3 wupA^PG31 wupA^PL87 y^+mDint2 z^58g z^ae(bx)
Constructs (14)
	P{YAL.wupA.2.6} P{YAL.wupA.2.7} P{YAL.wupA.4.7} P{YAL.wupA.4} P{YAL.wupA.IRE-0.3A} P{YAL.wupA.IRE-0.3B} P{YAL.wupA.IRE-0.3C} P{YAL.wupA.IRE-0.89} P{YAL.wupA.IRE-1.7} P{YAL.wupA.IRE-1.8} P{YAL.wupA.IRE-2.7} P{YAL.wupA.IRE-2.9} P{YAL.wupA.URE-0.5} P{YAL.wupA.URE-2.0}
Genes (17)
	bin Dpse\GA22384 Dpse\TpnI Ecol\lacZ gypsy\su(Hw)BR l(1)16Fe l(1)16Fm lmd Mef2 Mhc Su(z)2 tin Tm2 up wupA y z
Insertions (2)
	P{GawB}wupA^PG31 P{lacW}wupA^PL87
Sequence features (1)
	wupA_IRE-0.89
Transcripts (3)
	Ecol\lacZ^{4.7.wupA-Antp}RA Ecol\lacZ^{IRE-2.9.wupA-Antp}RA Ecol\lacZ^{URE-2.0.wupA-Antp}RA