Reference
Citation
Thibault, S.T., Singer, M.A., Miyazaki, W.Y., Milash, B., Dompe, N.A., Singh, C.M., Buchholz, R., Demsky, M., Fawcett, R., Francis-Lang, H.L., Ryner, L., Cheung, L.M., Chong, A., Erickson, C., Fisher, W.W., Greer, K., Hartouni, S.R., Howie, E., Jakkula, L., Joo, D., Killpack, K., Laufer, A., Mazzotta, J., Smith, R.D., Stevens, L.M., Stuber, C., Tan, L.R., Ventura, R., Woo, A., Zakrajsek, I., Zhao, L., Chen, F., Swimmer, C., Kopczynski, C., Duyk, G., Winberg, M.L., Margolis, J. (2004). A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac.  Nat. Genet. 36(3): 283--287.
FlyBase ID
FBrf0175002
Publication Type
Research paper
Abstract
With the availability of complete genome sequence for Drosophila melanogaster, one of the next strategic goals for fly researchers is a complete gene knockout collection. The P-element transposon, the workhorse of D. melanogaster molecular genetics, has a pronounced nonrandom insertion spectrum. It has been estimated that 87% saturation of the approximately 13,500-gene complement of D. melanogaster might require generating and analyzing up to 150,000 insertions. We describe specific improvements to the lepidopteran transposon piggyBac and the P element that enabled us to tag and disrupt genes in D. melanogaster more efficiently. We generated over 29,000 inserts resulting in 53% gene saturation and a more diverse collection of phenotypically stronger insertional alleles. We found that piggyBac has distinct global and local gene-tagging behavior from that of P elements. Notably, piggyBac excisions from the germ line are nearly always precise, piggyBac does not share chromosomal hotspots associated with P and piggyBac is more effective at gene disruption because it lacks the P bias for insertion in 5' regulatory sequences.
PubMed ID
PubMed Central ID
Related Publication(s)
Personal communication to FlyBase
Genomic mapping of Exelixis insertion collection. (Computer file)
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Note
Flying in the face of total disruption.
Hiesinger and Bellen, 2004, Nat. Genet. 36(3): 211--212 [FBrf0175000]
Associated Information
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Associated Files
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Genet.
    Title
    Nature Genetics
    Publication Year
    1992-
    ISBN/ISSN
    1061-4036 1546-1718
    Data From Reference
    Aberrations (10)
    Alleles (18)
    Genes (15)
    Datasets (4)
    Molecular Constructs (4)
    Molecular Segments (34)
    Natural transposons (1)
    Insertions (18944)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Experimental Tools (2)
    Transgenic Constructs (8)