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Reference Report
| Reference | |||
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| Citation | Thibault, S.T., Singer, M.A., Miyazaki, W.Y., Milash, B., Dompe, N.A., Singh, C.M., Buchholz, R., Demsky, M., Fawcett, R., Francis-Lang, H.L., Ryner, L., Cheung, L.M., Chong, A., Erickson, C., Fisher, W.W., Greer, K., Hartouni, S.R., Howie, E., Jakkula, L., Joo, D., Killpack, K., Laufer, A., Mazzotta, J., Smith, R.D., Stevens, L.M., Stuber, C., Tan, L.R., Ventura, R., Woo, A., Zakrajsek, I., Zhao, L., Chen, F., Swimmer, C., Kopczynski, C., Duyk, G., Winberg, M.L., Margolis, J. (2004). A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. Nat. Genet. 36(3): 283--287. (Export to RIS) | ||
| FlyBase ID | FBrf0175002 | ||
| Publication Type | Research paper | ||
| PubMed ID | 14981521 | ||
| PubMed Abstract | With the availability of complete genome sequence for Drosophila melanogaster, one of the next strategic goals for fly researchers is a complete gene knockout collection. The P-element transposon, the workhorse of D. melanogaster molecular genetics, has a pronounced nonrandom insertion spectrum. It has been estimated that 87% saturation of the approximately 13,500-gene complement of D. melanogaster might require generating and analyzing up to 150,000 insertions. We describe specific improvements to the lepidopteran transposon piggyBac and the P element that enabled us to tag and disrupt genes in D. melanogaster more efficiently. We generated over 29,000 inserts resulting in 53% gene saturation and a more diverse collection of phenotypically stronger insertional alleles. We found that piggyBac has distinct global and local gene-tagging behavior from that of P elements. Notably, piggyBac excisions from the germ line are nearly always precise, piggyBac does not share chromosomal hotspots associated with P and piggyBac is more effective at gene disruption because it lacks the P bias for insertion in 5' regulatory sequences. | ||
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| Related Publication(s) | |||
| Supplementary material | Supplementary Table 3. [FBrf0174228] Supplementary Table 2. [FBrf0174227] Supplementary Figure 1. [FBrf0174225] Supplementary Table 1: Hotspot Loci. [FBrf0174226] |
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| Personal communication to FlyBase | Genomic mapping of Exelixis insertion collection. (Computer file) Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] |
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Other Information
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| Secondary IDs | |||
| Language of Publication | English | ||
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Parent Publication
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| Publication Type | Journal | ||
| Abbreviation | Nat. Genet. | ||
| Title | Nature Genetics | ||
| Publication Year | 1992- | ||
| ISBN/ISSN | 1061-4036 1546-1718 | ||
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Data from Reference
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| Aberrations (10)
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| Alleles (20)
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| Constructs (46)
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| Genes (16)
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| Insertions (18951)
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| Libraries/Collections (4)
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| Natural transposons (1)
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