A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Reference Report

Reference
Citation Thibault, S.T., Singer, M.A., Miyazaki, W.Y., Milash, B., Dompe, N.A., Singh, C.M., Buchholz, R., Demsky, M., Fawcett, R., Francis-Lang, H.L., Ryner, L., Cheung, L.M., Chong, A., Erickson, C., Fisher, W.W., Greer, K., Hartouni, S.R., Howie, E., Jakkula, L., Joo, D., Killpack, K., Laufer, A., Mazzotta, J., Smith, R.D., Stevens, L.M., Stuber, C., Tan, L.R., Ventura, R., Woo, A., Zakrajsek, I., Zhao, L., Chen, F., Swimmer, C., Kopczynski, C., Duyk, G., Winberg, M.L., Margolis, J. (2004). A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac.  Nat. Genet. 36(3): 283--287. (Export to RIS)
FlyBase ID FBrf0175002
Publication Type Research paper
PubMed ID 14981521
PubMed Abstract With the availability of complete genome sequence for Drosophila melanogaster, one of the next strategic goals for fly researchers is a complete gene knockout collection. The P-element transposon, the workhorse of D. melanogaster molecular genetics, has a pronounced nonrandom insertion spectrum. It has been estimated that 87% saturation of the approximately 13,500-gene complement of D. melanogaster might require generating and analyzing up to 150,000 insertions. We describe specific improvements to the lepidopteran transposon piggyBac and the P element that enabled us to tag and disrupt genes in D. melanogaster more efficiently. We generated over 29,000 inserts resulting in 53% gene saturation and a more diverse collection of phenotypically stronger insertional alleles. We found that piggyBac has distinct global and local gene-tagging behavior from that of P elements. Notably, piggyBac excisions from the germ line are nearly always precise, piggyBac does not share chromosomal hotspots associated with P and piggyBac is more effective at gene disruption because it lacks the P bias for insertion in 5' regulatory sequences.
DOI
Related Publication(s)
Supplementary material Supplementary Table 3. [FBrf0174228]

Supplementary Table 2. [FBrf0174227]

Supplementary Figure 1. [FBrf0174225]

Supplementary Table 1: Hotspot Loci. [FBrf0174226]

Personal communication to FlyBase Genomic mapping of Exelixis insertion collection. (Computer file)
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]

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Language of Publication English
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Publication Type Journal
Abbreviation Nat. Genet.
Title Nature Genetics
Publication Year 1992-
ISBN/ISSN 1061-4036 1546-1718
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