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| Citation | Munoz, E., Cook, K. (2004.4.7). 'W3' confusion. (Export to RIS) | ||
| FlyBase ID | FBrf0178825 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
Date: Wed, 07 Apr 2004 17:19:01 \-0500
To: rd120@XXXXXXXXXXXXXXX From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: 'W3' confusion Cc: matthewkXXXXXXXXXXXXXXX, Enzo Muñoz <ermunozXXXXXXXXXXXXXXX> Hi Rachel-- Enzo Munoz and I have been corresponding about a case of confusing synonymy. I thought I'd summarize what we have figured out. Perhaps you can archive this note in case someone tries to work on these genes and alleles. There are two mutant strains called 'W3'. One of the mutated X chromosomes carries mutations in the run and unc loci, and the other strain carries a mutation in shakB. The relevant allele entries are run9 FBal0014854 uncW3a FBal0095151 shakB15 FBal0015588 The W3 chromosome carrying run9 and uncW3a was described in Lifschytz and Yakobovitz (Molec. Gen. Genet. 1978 161:275--284 = FBrf0032163). They described recombination experiments that mapped lethality both to the left of l(1)19Ec (=l(1)J9) and shakB (=l(1)R-9-29) and to the right of l(1)19Ec. They also showed that the Dp(1;Y)mal+-linked lethal l(1)YT14 failed to complement W3. Lindsley and Zimm (DIS 68: 110, 1990) cite unpublished results of George Lefevre, Jr. showing that 'l(1)W3b' is an allele of shakB. In this reference l(1)W3b was called pas15. (Passover is a synonym for shaking B.) In Lindsley and Zimm, 1992 (the Redbook), the synonym for shakB15 is l(1)N36, but I suspect 'N36' was simply a case of 'W3b' being written down illegibly. Enzo received both W3 lines from Lifschytz and maintained them as distinct stocks. Crosses made by Abe Schalet and Enzo showed that the run9 uncW3a stock failed to complement run4 (=l(1)LB19), run2 (=l(1)AA33), run18 (=l(1)17-26), run17-44, run19 (=l(1)17-169) and Df(1)16-3-53 for lethality and Df(1)17-351 for the unc phenotype, yet complemented shakB6 (=l(1)E81), shakB11 (=l(1)R-9-29), shakB19 (=l(1)17-189) and l(1)19Ec8 (=l(1)R-9-28). Enzo worked less with the shakB15 stock, but nevertheless showed that it failed to complement shakB6 (=l(1)E81) and shakB11 (=l(1)R-9-29), yet complemented Df(1)17-351. The distinction between the two W3 stocks must have been clear to Lifschytz and Lefevre, because references to the run9 uncW3a chromosomes use 'W3a' in allele designations and references to the shakB15 chromosomes use 'W3b'. Nevertheless, the distinction was blurred in both Lindsley and Zimm 1990 and 1992, where l(1)YT14 was listed as an allele of shakB (=shakB17) (presumably because both l(1)YT14 and shakB alleles failed to complement something called 'W3'!). In Figure 2 of Lifschytz and Yakobovitz, 1978, l(1)YT14 is shown mapping to the left of shakB, a position consistent with it being an allele of run, rather than an allele of unc. This position of l(1)YT14 was probably determined from complementation tests with deletions to the left of shakB. I recommend the following changes to FlyBase entries: (1) The shakB17 allele entry should be eliminated and a new allele entry created for l(1)YT14 under the run gene--perhaps as runYT14. (The l(1)YT14 chromosome is extinct, so the purpose of this is to correct a historical mistake and prevent confusion.) (2) l(1)W3 and l(1)W3b should be listed as synonyms for shakB15. Thanks! Kevin __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXXXXXXXXXXXXX |
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| Language of Publication | English | ||
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Data from Reference
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| Aberrations (2)
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| Alleles (11)
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| Genes (3)
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