HomeReference Report
| Reference | |
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| Citation | Parks, A. (2004.5.12). GAL4 constructs and insertions. |
| FlyBase ID | FBrf0178842 |
| Type of publication | Personal communication to FlyBase |
| Offprint Available | No |
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| Text of personal communication |
Date: Wed, 12 May 2004 15:19:38 \-0500
To: rd120XXXXXXXXXXXXXXX, flybase-updatesXXXXXXXXXXXXXXX, Annette Parks <annettep@XXXXXXXXXXXXXXX> From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: GAL4 constructs and insertions The following information was provided by Annette Parks of Exelixis, Inc. In P{ey3.5-GAL4.Exel}, the GAL4 gene was cloned into P{Express-ey3.5}. P{ey3.5-GAL4.Exel}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{ey3.5-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{ey3.5-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome insertion. In P{vgM-GAL4.Exel}, the GAL4 gene was cloned into P{Express-vgM}. P{vgM-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{vgM-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome insertion. In P{ey1x-GAL4.Exel}, the GAL4 gene was cloned into P{Express-ey1x}. P{ey1x-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{ey1x-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome insertion. In P{vgMQ-GAL4.Exel}, the GAL4 gene was cloned into P{Express-vgMQ}. P{vgMQ-GAL4.Exel}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{vgMQ-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{vgMQ-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome insertion. P{m4-GAL4.Exel} carries GAL4 driven by the E(spl) region transcript m4 (FBgn0002629) enhancer. The m4 enhancer was originally PCR amplified from a clone described in Singson et al., 1994 (Genes Dev. 8: 2058-2071; FBrf0076492) and cloned into KpnI and XhoI sites in the polylinker of pP{Express}. The enhancer fragment plus flanking pP{Express}sequence was cut out as a 0.5 kb SmaI-AscI fragment and cloned into the same sites in pP{Express-GAL4}. P{m4-GAL4.Exel}2 is a second chromosome insertion. P{E(spl)-GAL4.Exel} carries GAL4 driven by the E(spl) (FBgn0000591) enhancer. The enhancer fragment was PCR amplified from a clone described in Lecourtois & Schweisguth, 1995 (Genes Dev. 9: 2598-2608; FBrf0084115) and Kramatschek & Campos-Ortega, 1994 (Development 20: 815-26; FBrf0073637) and cloned into KpnI and XhoI sites in the pP{Express} polylinker. The enhancer fragment was cut out as a 1.1 kb SmaI-AscI fragment and cloned into pP{Express-GAL4}. P{E(spl)-GAL4.Exel}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{E(spl)-GAL4.Exel}3 is a third chromosome insertion. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXXXXXXXXXXXXX |
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| Language of publication | English |
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| Language of publication | English |
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Data from Reference
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| Alleles (7)
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| Constructs (6)
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| Genes (2)
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| Insertions (13)
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