Reference Report

Date: Wed, 12 May 2004 15:19:39 \-0500
To: rd120XXXXXXXXXXXXXXX, flybase-updatesXXXXXXXXXXXXXXX, Annette Parks
<annettep@XXXXXXXXXXXXXXX>
From: Kevin Cook <kcook@XXXXXXXXXXXXXXX>
Subject: InR constructs and insertions
The following information was provided by Annette Parks of Exelixis, Inc.
In P{GMR-InR.Exel}, InR (FBgn0013984) coding sequences corresponding to
accession AAC47458 were cloned into P{Express-glass}.
P{GMR-InR.Exel}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{GMR-InR.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
P{GMR-InR.Exel}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{UAS-InR.Exel}, InR (FBgn0013984) coding sequences corresponding to
accession AAC47458 were cloned into P{Express-UAS}.
P{UAS-InR.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
In P{sev-InR.Exel}, InR (FBgn0013984) coding sequences corresponding to
accession AAC47458 were cloned into either P{Express-sev1x} or
P{Express-sev3x}.
P{sev-InR.Exel}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{sev-InR.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
P{sev-InR.Exel}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{UAS-InR.del}, InR sequence deleted from Eco47III to XhoI to delete
most of the alpha subunit was cloned into P{Express-UAS}. This construct
mimics a construct expressing a constitutively active human InR described
by Ellis et al., 1987 (Mol Endocrin. 1:15-24).
P{UAS-InR.del}2 is a homozygous viable and fertile, second chromosome
insertion.
P{UAS-InR.del}3 is a homozygous viable and fertile, third chromosome insertion.
In P{GMR-InR.del}, InR sequence deleted from Eco47III to XhoI to delete
most of the alpha subunit was cloned into P{Express-glass}. This construct
mimics a construct expressing a constitutively active human InR described
by Ellis et al., 1987 (Mol Endocrin. 1:15-24).
P{GMR-InR.del}2 is a homozygous viable and fertile, second chromosome
insertion.
P{GMR-InR.del}3 is a homozygous viable and fertile, third chromosome insertion.
In P{UAS-InR.R418P}, InR sequences encoding a constitutively active R418P
variant protein were cloned into P{Express-UAS}. This fly variant protein
mimics the human K86P protein variant of Longo et al., 1993 (PNAS 90: 60-64).
P{UAS-InR.R418P}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{UAS-InR.R418P}2 is a homozygous viable and fertile, second chromosome
insertion.
In P{GMR-InR.R418P}, InR sequences encoding a constitutively active R418P
variant protein were cloned into P{Express-glass}. This fly variant
protein mimics the human K86P protein variant of Longo et al., 1993 (PNAS
90: 60-64).
P{GMR-InR.R418P}2 is a homozygous viable and fertile, second chromosome
insertion.
P{GMR-InR.R418P}3 is a third chromosome insertion.
In P{UAS-InR.K1409A}, InR sequences encoding a dominant negative K1409A
protein variant were cloned into P{Express-UAS}.
P{UAS-InR.K1409A}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{UAS-InR.K1409A}2 is a homozygous viable and fertile, second chromosome
insertion.
P{UAS-InR.K1409A}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{GMR-InR.K1409A}, InR sequences encoding a dominant negative K1409A
protein variant were cloned into P{Express-glass}.
P{GMR-InR.K1409A}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{GMR-InR.K1409A}2 is a homozygous viable and fertile, second chromosome
insertion.
P{GMR-InR.K1409A}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{UAS-InR.A1325D}, InR sequences encoding a constitutively active A1325D
variant protein were cloned into P{Express-UAS}. This fly variant protein
mimics the human V938D protein variant of Longo et al., 1992 (J Biol. Chem.
267:12416-9).
P{UAS-InR.A1325D}2 is a homozygous viable and fertile, second chromosome
insertion.
P{UAS-InR.A1325D}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{GMR-InR.A1325D}, InR sequences encoding a constitutively active A1325D
variant protein were cloned into P{Express-glass}. This fly variant
protein mimics the human V938D protein variant of Longo et al., 1992 (J
Biol. Chem. 267:12416-9).
P{GMR-InR.A1325D}2 is a homozygous viable and fertile, second chromosome
insertion.
P{GMR-InR.A1325D}3 is a homozygous viable and fertile, third chromosome
insertion.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-856-1213
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXXXXXXXXXXXXX