HomeReference Report
| Reference | |
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| Citation | Parks, A. (2004.5.12). lacZ constructs and insertions. |
| FlyBase ID | FBrf0178862 |
| Type of publication | Personal communication to FlyBase |
| Offprint Available | No |
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| Text of personal communication |
Date: Wed, 12 May 2004 15:23:46 \-0500
To: rd120XXXXXXXXXXXXXXX, flybase-updatesXXXXXXXXXXXXXXX, Annette Parks <annettep@XXXXXXXXXXXXXXX> From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: lacZ constructs and insertions The following information was provided by Annette Parks of Exelixis, Inc. In P{ey3.5-lacZ}, lacZ was cloned into EcoR1/Bgll2 sites in the polylinker of P{Express-ey3.5}. The lacZ gene was isolated in two fragments: EcoR1-Sac1 was from pC4-AUG-betagal (FBmc0000220) and is an in-frame fusion of the Adh leader to the 5' end of lacZ. The other half of lacZ was from pDCZA (a clone from Dan Curtis) and has the 3' end of lacZ directly fused to the Adh 3'UTR immediately following the stop codon. P{ey3.5-lacZ}2 is a homozygous viable and fertile, second chromosome insertion. P{ey3.5-lacZ}3 is a homozygous viable and fertile, third chromosome insertion. In P{vgM-lacZ.Exel}, lacZ was cloned into EcoR1/Bgll2 sites in the polylinker of P{Express-vgM}. The lacZ gene was isolated in two fragments: EcoR1-Sac1 was from pC4-AUG-betagal (FBmc0000220) and is an in-frame fusion of the ADH leader to the 5' end of lacZ. The other half of lacZ was from pDCZA (a clone from Dan Curtis) and has the 3' end of lacZ directly fused to the ADH 3'UTR immediately following the stop codon. P{vgM-lacZ.Exel}2 is a second chromosome insertion. P{vgM-lacZ.Exel}3 is a homozygous viable and fertile, third chromosome insertion. In P{GMR-UAS-lacZ}, a UAS-Hsp70TATA-lacZ fragment was cloned into P{Express-glass} in place of the Hsp70 TATA sequence of that vector using KpnI and SacII. The result was GMR-UAS-Hsp70TATA-lacZ. Both UAS and GMR can drive lacZ expression. P{GMR-UAS-lacZ}2 is a second chromosome insertion. P{GMR-UAS-lacZ}3 is a homozygous viable and fertile, third chromosome insertion. In P{Dll-UAS-lacZ}, a Distalless (FBgn0000157) enhancer was placed 5' of UAS-Hsp70TATA-lacZ in P{Express} using XhoI and KpnI. lacZ is expressed in the embryonic Dll pattern, but not in imaginal disks. UAS can also drive lacZ expression. P{Dll-UAS-lacZ}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. In P{dpp-lacZ.Exel.1} and P{dpp-lacZ.Exel.2}, the dpp disk enhancer described in St. Johnston et al., 1990 (Genes Dev. 4: 1114-1127; FBrf0051824) drives lacZ expression. In P{dpp-lacZ.Exel.1}, the relative orientation of the 3 kb enhancer fragment to lacZ matches the orientation of the enhancer and the dpp coding region in vivo and in P{TnJMB7} (FBtp0004773) described in Masucci et al., 1990 (Genes. Dev. 4: 2011-2023; FBrf0051842). In P{dpp-lacZ.Exel.2}, the enhancer is in the opposite orientation and matches that in P{TnJMB2} (FBtp0004772) also described in Masucci et al. P{dpp-lacZ.Exel.1}2 is a homozygous viable and fertile, second chromosome insertion. P{dpp-lacZ.Exel.2}1is an X chromosome insertion. P{dpp-lacZ.Exel.2}2 is a homozygous viable and fertile, second chromosome insertion. P{dpp-lacZ.Exel.2}3 is a homozygous viable and fertile, third chromosome insertion. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXXXXXXXXXXXXX |
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| Language of publication | English |
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| Language of publication | English |
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Data from Reference
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| Alleles (7)
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| Constructs (6)
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| Genes (2)
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| Insertions (11)
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