HomeReference Report
| Reference | |
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| Citation | Parks, A. (2004.6). P{UAS-Pten.dsRNA.Exel} construct and insertions. |
| FlyBase ID | FBrf0178905 |
| Type of publication | Personal communication to FlyBase |
| Offprint Available | No |
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| Text of personal communication |
Date: Thu, 05 Aug 2004 17:07:51 \-0500
To: flybase-updates@XXXXXXXXXXXXXXX From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: P{UAS-Pten.dsRNA.Exel} construct and insertions Cc: 'Annette Parks':; The following information was provided by Annette Parks of Exelixis, Inc (6/04). P{UAS-Pten.dsRNA.Exel} is a transgene construct which generates dsRNA transcripts for RNA interference of the Pten gene. Nucleotides 295 through 819 from Pten sequence AF201905 were cloned in the forward and reverse directions into pJM1084 (the hairpin vector described in Reichhart et al. 2002, genesis 34:160-164; FBrf0152370). The cloned sequences differed from AF201905 by an A to C change at nucleotide 423. P{UAS-Pten.dsRNA.Exel}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{UAS-Pten.dsRNA.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{UAS-Pten.dsRNA.Exel}3 is a homozygous viable and fertile, third chromosome insertion. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXXXXXXXXXXXXX |
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| Language of publication | English |
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| Language of publication | English |
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| Alleles (1)
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| Constructs (1)
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| Genes (1)
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| Insertions (3)
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