Reference Report

Reference
Citation	Bauer, R., Lehmann, C., Martini, J., Eckardt, F., Hoch, M. (2004). Gap junction channel protein innexin 2 is essential for epithelial morphogenesis in the Drosophila embryo. Mol. Biol. Cell 15(6): 2992--3004. (Export to RIS)
FlyBase ID	FBrf0179129
Publication Type	Research paper
PubMed ID	15047872
PubMed Abstract	Direct communication of neighboring cells by gap junction channels is essential for the development of tissues and organs in the body. Whereas vertebrate gap junctions are composed of members of the connexin family of transmembrane proteins, in invertebrates gap junctions consist of Innexin channel proteins. Innexins display very low sequence homology to connexins. In addition, very little is known about their cellular role during developmental processes. In this report, we examined the function and the distribution of Drosophila Innexin 2 protein in embryonic epithelia. Both loss-of-function and gain-of-function innexin 2 mutants display severe developmental defects due to cell death and a failure of proper epithelial morphogenesis. Furthermore, immunohistochemical analyses using antibodies against the Innexins 1 and 2 indicate that the distribution of Innexin gap junction proteins to specific membrane domains is regulated by tissue specific factors. Finally, biochemical interaction studies together with genetic loss- and gain-of-function experiments provide evidence that Innexin 2 interacts with core proteins of adherens and septate junctions. This is the first study, to our knowledge, of cellular distribution and protein-protein interactions of an Innexin gap junctional channel protein in the developing epithelia of Drosophila.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Alleles (11)
	arm⁴ BacA\p35^Scer\UAS.cHa cora⁵ Inx2^G0016 Inx2^Scer\UAS.cBa Inx2^{Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^prd.RG1 shg² T:Avic\GFP^EGFP w^+mC
Constructs (5)
	P{GAL4-prd.F} P{matα4-GAL-VP16} P{UAS-inx2.B} P{UAS-inx2.EGFP} P{UAS-p35.H}
Genes (11)
	arm BacA\p35 cora crb en Inx2 ogre Scer\GAL4 shg T:Hsim\VP16 w
Insertions (1)
	P{lacW}Inx2^G0016