Reference Report

Reference
Citation	Bellen, H.J., Levis, R.W., Liao, G., He, Y., Carlson, J.W., Tsang, G., Evans-Holm, M., Hiesinger, P.R., Schulze, K.L., Rubin, G.M., Hoskins, R.A., Spradling, A.C. (2004). The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. Genetics 167(2): 761--781.
FlyBase ID	FBrf0179132
Type of publication	Research paper
Offprint Available	No
External Crossreferences
PubMed ID	15238527
PubMed Abstract	The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each Drosophila gene by the insertion of a single transposable element. As part of this effort, transposons in >30,000 fly strains were localized and analyzed relative to predicted Drosophila gene structures. Approximately 6300 lines that maximize genomic coverage were selected to be sent to the Bloomington Stock Center for public distribution, bringing the size of the BDGP gene disruption collection to 7140 lines. It now includes individual lines predicted to disrupt 5362 of the 13,666 currently annotated Drosophila genes (39%). Other lines contain an insertion at least 2 kb from others in the collection and likely mutate additional incompletely annotated or uncharacterized genes and chromosomal regulatory elements. The remaining strains contain insertions likely to disrupt alternative gene promoters or to allow gene misexpression. The expanded BDGP gene disruption collection provides a public resource that will facilitate the application of Drosophila genetics to diverse biological problems. Finally, the project reveals new insight into how transposons interact with a eukaryotic genome and helps define optimal strategies for using insertional mutagenesis as a genomic tool.
Biosis	2004.363321
Zoological record
Associated Information
Comments
Text of personal communication
Associated files
Related Publications
Computer file	[title not yet available] Gene Disruption Project members, 2001-, [title not yet available] [FBrf0132177] Genomic mapping of P{Mae-UAS.6.11} insertion collection. Gene Disruption Project members and Merriam, 2005, Genomic mapping of P{Mae-UAS.6.11} insertion collection. [FBrf0188778]
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Other Reference Information
Secondary IDs
Language of publication	English
Additional language(s) of abstract
ISBN
Place of publication
Published In
Abbreviation	Genetics
Title	Genetics
Authors
Volume range	1-
Year range	1916-
Page range
Publisher
Place of publication	Austin, TX, etc
Language of publication	English
ISBN/ISSN	0016-6731
CODEN	GENTAE
Data from Reference
Aberrations
	In(2LR)O
Alleles
	Abd-B⁰⁵⁶⁴⁹ CG32767^BG01357 tun^KG10308 y^+mDint2
Balancers
	CyO CyO-BG00000 CyO-EY00000 CyO-P{SUPor-P}RR1
Genes
	ATPCL Abd-B Acon Acp65Aa Adf1 CG11033 CG11367 CG14450 CG14709 CG3036 CG3104 CG31475 CG31522 CG31626 CG31690 CG31849 CG32027 CG32529 CG32767 CG4663 CG4670 CG5287 CG5458 CG5555 CG7582 CG8249 CG8315 CG8320 CG8677 CG8678 CG8709 CG9216 CG9243 CG9894 Cpr65Au Cpr65Av Crc Dp Gdh Gli GstS1 His3:CG31613 His4:CG31611 HmgD Hr39 Indy Lcp65Ab1 Lcp65Ab2 Lcp65Ac Lcp65Ad Lcp65Ae Lcp65Af Lcp65Ag1 Lcp65Ag2 Lcp65Ag3 Lcp65AΨ Men Pabp2 Pld RhoGAP18B RhoL Sin3A Slh Ten-m Thor amn apt bin3 bun cher cpo daw esg exba fas fok for fru grp heph kermit kis l(2)01289 lama lola mub neb oaf pum sra tara th tlk trx ttk tun wun y
Insertions
	Too many objects to list.
Other
	PBac{3HPy⁺} PBac{5HPw⁺} PBac{GAL4D,EYFP} P{EPgy2} P{EP} P{GT1} P{Mae-UAS.6.11} P{PZ} P{SUPor-P} P{lacW} P{wHy}