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Reference Report
| Reference | |||
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| Citation | Bellen, H.J., Levis, R.W., Liao, G., He, Y., Carlson, J.W., Tsang, G., Evans-Holm, M., Hiesinger, P.R., Schulze, K.L., Rubin, G.M., Hoskins, R.A., Spradling, A.C. (2004). The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. Genetics 167(2): 761--781. (Export to RIS) | ||
| FlyBase ID | FBrf0179132 | ||
| Publication Type | Research paper | ||
| PubMed ID | 15238527 | ||
| PubMed Abstract | The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each Drosophila gene by the insertion of a single transposable element. As part of this effort, transposons in >30,000 fly strains were localized and analyzed relative to predicted Drosophila gene structures. Approximately 6300 lines that maximize genomic coverage were selected to be sent to the Bloomington Stock Center for public distribution, bringing the size of the BDGP gene disruption collection to 7140 lines. It now includes individual lines predicted to disrupt 5362 of the 13,666 currently annotated Drosophila genes (39%). Other lines contain an insertion at least 2 kb from others in the collection and likely mutate additional incompletely annotated or uncharacterized genes and chromosomal regulatory elements. The remaining strains contain insertions likely to disrupt alternative gene promoters or to allow gene misexpression. The expanded BDGP gene disruption collection provides a public resource that will facilitate the application of Drosophila genetics to diverse biological problems. Finally, the project reveals new insight into how transposons interact with a eukaryotic genome and helps define optimal strategies for using insertional mutagenesis as a genomic tool. | ||
| DOI | |||
| Related Publication(s) | |||
| Personal communication to FlyBase | (Computer file) Gene Disruption Project members, 2001-, (Computer file) [FBrf0132177] Insertion site data for additional GT1 (BG) lines. Levis et al., 2011.12.16, Insertion site data for additional GT1 (BG) lines. [FBrf0217018] Genomic mapping of P{Mae-UAS.6.11} insertion collection. (Computer file) Gene Disruption Project members and Merriam, 2005, Genomic mapping of P{Mae-UAS.6.11} insertion collection. (Computer file) [FBrf0188778] |
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Insertions
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| Secondary IDs | |||
| Language of Publication | English | ||
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Parent Publication
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| Publication Type | Journal | ||
| Abbreviation | Genetics | ||
| Title | Genetics | ||
| Publication Year | 1916- | ||
| ISBN/ISSN | 0016-6731 | ||
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Data from Reference
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| Aberrations (1)
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| Alleles (4)
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| Balancers (4)
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| Constructs (11)
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| Genes (100)
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| Insertions (40107)
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| Libraries/Collections (12)
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