Reference Report

Reference
Citation	Blanchette, M., Labourier, E., Green, R.E., Brenner, S.E., Rio, D.C. (2004). Genome-wide analysis reveals an unexpected function for the Drosophila splicing factor U2AF50 in the nuclear export of intronless mRNAs. Mol. Cell 14(6): 775--786. (Export to RIS)
FlyBase ID	FBrf0179139
Publication Type	Research paper
PubMed ID	15200955
PubMed Abstract	The protein factor U2AF is an essential component required for pre-mRNA splicing. Mutations identified in the S. pombe large U2AF subunit were used to engineer transgenic Drosophila carrying temperature-sensitive U2AF large subunit alleles. Mutant recombinant U2AF heterodimers showed reduced polypyrimidine tract RNA binding at elevated temperatures. Genome-wide RNA profiling comparing wild-type and mutant strains identified more than 400 genes differentially expressed in the dU2AF50 mutant flies grown at the restrictive temperature. Surprisingly, almost 40% of the downregulated genes lack introns. Microarray analyses revealed that nuclear export of a large number of intronless mRNAs is impaired in Drosophila-cultured cells RNAi knocked down for dU2AF50. Immunopurification of nuclear RNP complexes showed that dU2AF50 associates with intronless mRNAs. These results reveal an unexpected role for the splicing factor dU2AF50 in the nuclear export of intronless mRNAs.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell
Title	Molecular Cell
Publication Year	1997-
ISBN/ISSN	1097-2765 1097-4164
Data from Reference
Alleles (6)
	U2af50^+mBa U2af50^D150A.E151A U2af50^D204N.ts U2af50^S284Y.ts U2af50^T160I U2af50^XR15
Constructs (5)
	P{U2af50.+mBa} P{U2af50.D150A.E151A} P{U2af50.D204N.ts} P{U2af50.S284Y.ts} P{U2af50.T160I}
Genes (44)
	Ag5r2 brk CG4783 CG6503 CG7630 CG8628 CG13323 CG15784 CG16712 CG17571 CG18404 CG18508 CoVa Cp16 Cp18 Cp19 Cp36 Cp38 CycB3 cype Cys Gip Jon65Ai Jon99Fi Jon99Fii mag Mpp6 MtnC Neb-cGP noi Nplp2 Obp99c PGRP-SC1a PGRP-SC1b Prp38 RpL32 slp1 U2af50 Vm32E yip7 Yp3 βTry γTry λTry
Insertions (7)
	P{U2af50.D204N.ts}1 P{U2af50.D204N.ts}2 P{U2af50.D204N.ts}3 P{U2af50.D204N.ts}4 P{U2af50.S284Y.ts}1 P{U2af50.S284Y.ts}2 P{U2af50.S284Y.ts}3