Reference Report

Reference
Citation	Bruckner, K., Kockel, L., Duchek, P., Luque, C.M., Rorth, P., Perrimon, N. (2004). The PDGF/VEGF receptor controls blood cell survival in Drosophila. Dev. Cell 7(1): 73--84. (Export to RIS)
FlyBase ID	FBrf0179147
Publication Type	Research paper
PubMed ID	15239955
PubMed Abstract	The Drosophila PDGF/VEGF receptor (PVR) has known functions in the guidance of cell migration. We now demonstrate that during embryonic hematopoiesis, PVR has a role in the control of antiapoptotic cell survival. In Pvr mutants, a large fraction of the embryonic hemocyte population undergoes apoptosis, and the remaining blood cells cannibalistically phagocytose their dying peers. Consequently, total hemocyte numbers drop dramatically during embryogenesis, and large aggregates of engorged macrophages carrying multiple apoptotic corpses form. Hemocyte-specific expression of the pan-caspase inhibitor p35 in Pvr mutants eliminates hemocyte aggregates and restores blood cell counts and morphology. Additional rescue experiments suggest involvement of the Ras pathway in PVR-mediated blood cell survival. In cell culture, we demonstrate that PVR directly controls survival of a hemocyte cell line. This function of PVR shows striking conservation with mammalian hematopoiesis and establishes Drosophila as a model to study hematopoietic cell survival in development and disease.
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Supplementary material	[FBrf0200152]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Alleles (20)
	Avic\GFP^{E.Scer\UAS.T:Uuuu\Myr4} BacA\p35^Scer\UAS.cHa Ecol\lacZ^{Scer\UAS.T:SV40\nls2} Egfr^DN.Scer\UAS Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} Pvr¹ Pvr⁴ Pvr^dsRNA.3'UTR Pvr^dsRNA.int Pvr^tub.PB Pvr^{ΔC.Scer\UAS.T:Hsap\EFNB1-SP,T:Ivir\HA1} Pvr^{λ.Scer\UAS.T:Hsap\MYC} Ras85D^N17.Scer\UAS Ras85D^V12.Scer\UAS Scer\GAL4^lz-gal4 Scer\GAL4^srp.Hemo Scer\GAL4^twi.PG T:Hsap\EFNB1^SP W^{Ala5.Scer\UAS}
Constructs (14)
	P{Dp110^D954A} P{Dp110-CAAX} P{GAL4-twi.G} P{srp.Hemo-GAL4} P{tub-Pvr.B} P{UAS-Egfr.DN} P{UAS-lacZ.NZ} P{UAS-p35.H} P{UAS-Pvr.ΔC.HA} P{UAS-Pvr.λ} P{UAS-Ras85D.N17} P{UAS-Ras85D.V12} P{UAS-srcEGFP} P{UAS-W.Ala5}
Genes (14)
	Avic\GFP BacA\p35 crq decay Ecol\lacZ Egfr lz Pi3K92E Pvr Pxn Ras85D Scer\GAL4 T:Hsap\EFNB1 W
Insertions (1)
	P{GawB}lz^gal4