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Citation
Cakouros, D., Daish, T.J., Kumar, S. (2004). Ecdysone receptor directly binds the promoter of the Drosophila caspase dronc, regulating its expression in specific tissues.  J. Cell Biol. 165(5): 631--640.
FlyBase ID
FBrf0179151
Publication Type
Research paper
Abstract

The steroid hormone ecdysone regulates moulting, cell death, and differentiation during insect development. Ecdysone mediates its biological effects by either direct activation of gene transcription after binding to its receptor EcR-Usp or via hierarchical transcriptional regulation of several primary transcription factors. In turn, these transcription factors regulate the expression of several downstream genes responsible for specific biological outcomes. DRONC, the Drosophila initiator caspase, is transcriptionally regulated by ecdysone during development. We demonstrate here that the dronc promoter directly binds EcR-Usp. We further show that mutation of the EcR-Usp binding element (EcRBE) reduces transcription of a reporter and abolishes transactivation by an EcR isoform. We demonstrate that EcRBE is required for temporal regulation of dronc expression in response to ecdysone in specific tissues. We also uncover the participation of a putative repressor whose function appears to be coupled with EcR-Usp. These results indicate that direct binding of EcR-Usp is crucial for controlling the timing of dronc expression in specific tissues.

PubMed ID
PubMed Central ID
PMC2172386 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Alleles (5)
    Genes (12)
    Cell Lines (1)
    Natural transposons (1)
    Experimental Tools (1)
    Transgenic Constructs (4)