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Reference
Citation	Franco, B., Bogdanik, L., Bobinnec, Y., Debec, A., Bockaert, J., Parmentier, M.L., Grau, Y. (2004). Shaggy, the homolog of glycogen synthase kinase 3, controls neuromuscular junction growth in Drosophila. J. Neurosci. 24(29): 6573--6577.
FlyBase ID	FBrf0179211
Type of publication	Research paper
Offprint Available	Yes
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PubMed ID	15269269
PubMed Abstract	A protein-trap screen using the Drosophila neuromuscular junction (NMJ) as a model synapse was performed to identify genes that control synaptic structure or plasticity. We found that Shaggy (Sgg), the Drosophila homolog of the mammalian glycogen synthase kinases 3 alpha and beta, two serine-threonine kinases, was concentrated at this synapse. Using various combinations of mutant alleles of shaggy, we found that Shaggy negatively controlled the NMJ growth. Moreover, tissue-specific expression of a dominant-negative Sgg indicated that this kinase is required in the motoneuron, but not in the muscle, to control NMJ growth. Finally, we show that Sgg controlled the microtubule cytoskeleton dynamics in the motoneuron and that Futsch, a microtubule-associated protein, was required for Shaggy function on synaptic growth.
Biosis	2004.375200
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Language of publication	English
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Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Authors
Volume range	1-
Year range	1981-
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Place of publication	New York
Language of publication	English
ISBN/ISSN	0270-6474
CODEN	JNRSDS
Data from Reference
Aberrations (1)
	Df(1)64j4
Alleles (11)
	Avic\GFP^sgg-ZCL1626 Scer\GAL4^BG380 Scer\GAL4^Mhc.PW Scer\GAL4^OK6 Scer\GAL4^elav-C155 futsch^K68 futsch^N94 sgg¹⁰ sgg^{A81T.Scer\UAS} sgg^ZCL1626 sgg^mR10
Constructs (4)
	P{GAL4-Mhc.W} P{PTT-un1} P{UAS-sgg.A81T} P{mini10}
Genes (4)
	Avic\GFP Scer\GAL4 futsch sgg
Insertions (4)
	P{GAL4}BG380 P{GawB}OK6 P{GawB}elav^C155 P{PTT-un1}sgg^ZCL1626