Reference Report

Reference
Citation	Koh, T.W., Verstreken, P., Bellen, H.J. (2004). Dap160/Intersectin acts as a stabilizing scaffold required for synaptic development and vesicle endocytosis. Neuron 43(2): 193--205. (Export to RIS)
FlyBase ID	FBrf0179283
Publication Type	Research paper
PubMed ID	15260956
PubMed Abstract	We describe the isolation of mutations in dynamin-associated protein 160 kDa (dap160), the Drosophila homolog of intersectin, a putative adaptor for proteins involved in endocytosis, cytoskeletal regulation, and signaling. We show that partial loss-of-function mutants display temperature-sensitive (ts) paralysis, whereas null mutants show ts defects in endocytosis. Loss-of-function mutants exhibit bouton overgrowth at larval neuromuscular junctions (NMJs), but evoked neurotransmission is normal. Mutant NMJs show a mild endocytic defect at 22 degrees C, which is strongly enhanced at 34 degrees C. The levels of dynamin, synaptojanin and endophilin are severely reduced in dap160 mutant NMJs, suggesting that Dap160 serves to stabilize an endocytic macromolecular complex. Electron microscopy reveals fewer vesicles, aberrant large vesicles, and an accumulation of endocytic intermediates at active and periactive zones in mutant terminals. Our data suggest that Dap160, like dynamin, is involved in synaptic vesicle retrieval at active and periactive zones.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Aberrations (4)
	Df(2L)bur-K1 Df(2L)DS8 Df(2L)DS9 Df(2L)Rev18
Alleles (19)
	CG9253^EY02880 CG9253^KG05120 CG9253^Δ6 Dap160^EMS Dap160^EP2468 Dap160^EP2543 Dap160^Scer\UAS.cKa Dap160^unspecified Dap160^Δ1 Dap160^Δ2 Dap160^Δ3 Dap160^Δ4 Dap160^Δ5 Dap160^Δ6 l(2)05287^Δ3 nwk² Scer\GAL4^elav-C155 Scer\GAL4^αTub84B.PL shi¹
Constructs (2)
	P{tubP-GAL4} P{UAS-Dap160.K}
Genes (24)
	CG9253 CG9257 CG9259 CG9265 CG9266 CG12050 Csp Dap160 dlg1 endoA futsch GluRIIA Itgβν l(2)05287 lap Nhe2 nwk ppk13 Scer\GAL4 shi Syn synj Syt1 tadr
Insertions (3)
	P{EP}Dap160^EP2468 P{EP}Dap160^EP2543 P{GawB}elav^C155