Reference Report

Reference
Citation	Martinez-Campos, M., Basto, R., Baker, J., Kernan, M., Raff, J.W. (2004). The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosis. J. Cell Biol. 165(5): 673--683. (Export to RIS)
FlyBase ID	FBrf0179327
Publication Type	Research paper
PubMed ID	15184400
PubMed Abstract	Centrosomes consist of a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Proteins that contain a Pericentrin/AKAP450 centrosomal targeting (PACT) domain have been implicated in recruiting several proteins to the PCM. We show that the only PACT domain protein in Drosophila (the Drosophila pericentrin-like protein [D-PLP]) is associated with both the centrioles and the PCM, and is essential for the efficient centrosomal recruitment of all six PCM components that we tested. Surprisingly, however, all six PCM components are eventually recruited to centrosomes during mitosis in d-plp mutant cells, and mitosis is not dramatically perturbed. Although viable, d-plp mutant flies are severely uncoordinated, a phenotype usually associated with defects in mechanosensory neuron function. We show that the sensory cilia of these neurons are malformed and the neurons are nonfunctional in d-plp mutants. Moreover, the flagella in mutant sperm are nonmotile. Thus, D-PLP is essential for the formation of functional cilia and flagella in flies.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(3L)Brd15
Alleles (6)
	cp309⁵ cp309^{PACT.Ubi-p63E.T:Avic\GFP} cp309^s2172 cp309^unspecified Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^elav-C155
Constructs (2)
	P{UAS-mCD8::GFP.L} P{Ubi-p63E-GFP-cp309.PACT}
Genes (16)
	Avic\GFP cnn cp309 His3 Map60 Mmus\Cd8a Or71a Scer\GAL4 T:Avic\GFP tacc αTub67C αTub84B αTub84D αTub85E γTub23C γTub37C
Insertions (2)
	P{GawB}elav^C155 P{lacW}cp309^s2172
Natural transposons (1)
	P-element