Reference Report

Reference
Citation	Raymond, K., Bergeret, E., Avet-Rochex, A., Griffin-Shea, R., Fauvarque, M.O. (2004). A screen for modifiers of RacGAP(84C) gain-of-function in the Drosophila eye revealed the LIM kinase Cdi/TESK1 as a downstream effector of Rac1 during spermatogenesis. J. Cell Sci. 117(13): 2777--2789. (Export to RIS)
FlyBase ID	FBrf0179402
Publication Type	Research paper
PubMed ID	15169836
PubMed Abstract	In Drosophila, RotundRacGAP/RacGAP(84C) is critical to retinal organisation and spermatogenesis. We show that eye-directed expression of RacGAP(84C) or its GTPase activating protein (GAP) domain induces a dominant rough eye phenotype which we used as a starting point in a gain-of-function screen to identify new partners of RacGAP(84C). Proteins known to function in Ras, Rho and Rac signalling were identified confirming the essential role of RacGAP(84C) in crosstalk between GTPases. Other potential RacGAP(84C) partners identified by the screen are implicated in signal transduction, DNA remodelling, cytoskeletal organisation, membrane trafficking and spermatogenesis. This latter class includes the serine/threonine kinase Center divider (Cdi), which is homologous to the human LIM kinase, Testis specific kinase 1 (TESK1), involved in cytoskeleton control through Cofilin phosphorylation. Eye-directed expression of cdi strongly suppressed the phenotypes induced by either RacGAP(84C) gain-of-function or by the dominant negative form of Rac1, Rac1N17. These results are consistent with Cdi being a specific downstream target of Rac1. We showed that Rac1 and cdi are both expressed in Drosophila testis and that homozygous Rac1 mutants exhibit poor fertility that is further reduced by introducing a cdi loss-of-function mutation in trans. Thus, results from a misexpression screen in the eye led us to a putative novel Rac1-Cdi-Cofilin pathway, regulated by RacGAP(84C), coordinating Drosophila spermatogenesis.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Aberrations (2)
	Df(3L)st-e4 Df(3R)rn20
Alleles (43)
	Akap200^EP2072 Akap200^EP2254 babos^EP827 bchs^EP2299 C3G^EP1613 C3G^{Scer\UAS.T:Hsap\MYC} Cdc42^N17.Scer\UAS cdi^EP3319 cdi^R47 CG2017^EP3503 CG5261^EP816 CG6701^EP2054 cindr^EP3700 ckd^EP714 ckd^EP3139 cpo^EP3395 cpo^EP3608 dom^EP2371 Dp1^EP2422 Ecol\lacZ^cdi-242 EP474^EP474 grp^EP587 hppy^EP2445 kis^EP563 MESR4^EP386 numb^EP2455 pbl⁵ pbl^EP3415 poe^EP349 poe^EP737 Rac1^J11 Rac1^N17.Scer\UAS Rac2^Δ RacGAP84C^GAP.Scer\UAS RacGAP84C^Scer\UAS.cRa RacGAP84C^Zn.Scer\UAS Ras85D^N17.Scer\UAS rho-6^EP2023 RhoGEF64C^EP3035 RhoGEF64C^EP3322 Scer\GAL4^GMR.PU SNF4Aγ^EP648 Traf6^EP325
Constructs (8)
	P{GMR-GAL4.U} P{UAS-C3G.T:Myc} P{UAS-Cdc42.N17} P{UAS-Rac1.N17} P{UAS-Ras85D.N17} P{UAS-rnRacGAP.GAP} P{UAS-rnRacGAP.R} P{UAS-rnRacGAP.Zn}
Genes (37)
	Akap200 babos bchs C3G Cdc42 cdi CG2017 CG5261 CG6701 CG8740 cindr ckd cpo dom Dp1 Ecol\lacZ EP474 grp hppy kis MESR4 Myo31DF numb pbl poe Rac1 Rac2 RacGAP84C Ras85D rho-6 RhoGEF64C rn Scer\GAL4 SNF4Aγ TMS1 Traf6 wun2
Insertions (34)
	P{EP}Akap200^EP2072 P{EP}Akap200^EP2254 P{EP}babos^EP827 P{EP}bchs^EP2299 P{EP}C3G^EP1613 P{EP}cdi^EP3319 P{EP}CG2017^EP3503 P{EP}CG5261^EP816 P{EP}CG6701^EP2054 P{EP}cindr^EP3700 P{EP}ckd^EP714 P{EP}ckd^EP3139 P{EP}cpo^EP3395 P{EP}cpo^EP3608 P{EP}dom^EP2371 P{EP}Dp1^EP2422 P{EP}EP474^EP474 P{EP}EP2233 P{EP}EP2491 P{EP}fax^EP807 P{EP}GIIIspla2^EP325 P{EP}grp^EP587 P{EP}hppy^EP2445 P{EP}kis^EP563 P{EP}MESR4^EP386 P{EP}numb^EP2455 P{EP}pbl^EP3415 P{EP}poe^EP349 P{EP}poe^EP737 P{EP}rho-6^EP2023 P{EP}RhoGEF64C^EP3035 P{EP}RhoGEF64C^EP3322 P{EP}SNF4Aγ^EP648 P{EP}wun^EP652
Transcripts (1)
	Ecol\lacZ^cdi-242RA