|Citation||Sawatsubashi, S., Maki, A., Ito, S., Shirode, Y., Suzuki, E., Zhao, Y., Yamagata, K., Kouzmenko, A., Takeyama, K., Kato, S. (2004). Ecdysone receptor-dependent gene regulation mediates histone poly(ADP-ribosyl)ation. Biochem. Biophys. Res. Commun. 320(1): 268--272. (Export to RIS)|
|Publication Type||Research paper|
|PubMed Abstract||While the ecdysone dependency of puff formation in giant polytene chromosomes from fly salivary glands has been well documented, the molecular mechanisms underlying this process remain unknown. However, it does appear to involve chromatin remodeling and modification mediated by ecdysone receptor (EcR). As Drosophila poly(ADP-ribose) polymerase (dPARP) has recently been reported to be involved in ecdysone-induced puff formation, we decided to test the possible role of dPARP in ligand-induced dEcR transactivation in an insect system. dPARP co-activated the ligand-induced transactivation function of EcR in the insect cell line S2, and appeared to physically interact with EcR in a ligand-dependent manner. ChIP analysis of an EcR target gene promoter revealed ligand-dependent recruitment of dPARP with poly(ADP-ribosyl)ation of histones in the EcR binding site and, surprisingly, also in a distal region of the promoter. Our results indicated that EcR-mediated gene regulation may be coupled with chromatin modification through poly(ADP-ribosyl)ation.|
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|Language of Publication||English|
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|Abbreviation||Biochem. Biophys. Res. Commun.|
|Title||Biochemical and Biophysical Research Communications|
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