Reference Report

Reference
Citation	Schulz, C., Kiger, A.A., Tazuke, S.I., Yamashita, Y.M., Pantalena-Filho, L.C., Jones, D.L., Wood, C.G., Fuller, M.T. (2004). A misexpression screen reveals effects of bag-of-marbles and TGF beta class signaling on the Drosophila male germ-line stem cell lineage. Genetics 167(2): 707--723. (Export to RIS)
FlyBase ID	FBrf0179436
Publication Type	Research paper
PubMed ID	15238523
PubMed Abstract	Male gametes are produced throughout reproductive life by a classic stem cell mechanism. However, little is known about the molecular mechanisms for lineage production that maintain male germ-line stem cell (GSC) populations, regulate mitotic amplification divisions, and ensure germ cell differentiation. Here we utilize the Drosophila system to identify genes that cause defects in the male GSC lineage when forcibly expressed. We conducted a gain-of-function screen using a collection of 2050 EP lines and found 55 EP lines that caused defects at early stages of spermatogenesis upon forced expression either in germ cells or in surrounding somatic support cells. Most strikingly, our analysis of forced expression indicated that repression of bag-of-marbles (bam) expression in male GSC is important for male GSC survival, while activity of the TGF beta signal transduction pathway may play a permissive role in maintenance of GSCs in Drosophila testes. In addition, forced activation of the TGF beta signal transduction pathway in germ cells inhibits the transition from the spermatogonial mitotic amplification program to spermatocyte differentiation.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (71)
	Avic\GFP^Scer\UAS.cUa bam^{-898+133.T:Avic\GFP} bam^EP667 bam^{Scer\UAS.P\T.T:Avic\GFP} beag^EP3260 bun^EP2622 CG3308^EP1230 CG5376^EP709 CG5807^EP3455 CG8833^EP3365 CG17646^EP2506 CG31176^EP3662 corto^EP775 Csp^EP659 CycH^EP3658 dally^EP581 dpp^e90 dpp^EP2232 dpp^hr56 dpp^Scer\UAS.cSa dpp^unspecified EP303^EP303 EP416^EP416 EP569^EP569 EP605^EP605 EP639^EP639 EP917^EP917 EP1388^EP1388 EP1457^EP1457 EP1547^EP1547 EP2031^EP2031 EP2182^EP2182 EP2522^EP2522 EP3015a^EP3015a EP3269^EP3269 EP3281^EP3281 EP3289^EP3289 EP3367^EP3367 EP3582^EP3582 EP3645^EP3645 EP3683^EP3683 fabp^EP3252 gish^EP3652 glec^EP410 heph^EP3204 heph^Scer\UAS.cSa l(3)L4092^EP3690 lama^EP3629 Lmpt^EP610 Lpin^EP2431 mam^EP2198 Men^EP500 Nrx-IV^EP809 numb^EP2505 Pdk1^EP3091 sax^unspecified Scer\GAL4^{nos.UTR.T:Hsim\VP16} Scer\GAL4^ptc-559.1 SNF4Aγ^EP648 SNF4Aγ^EP3015b Spec2^EP3740 spin^EP2363 sqd^EP746 T:Avic\GFP^S65C tkv^{Q253D.Scer\UAS.cNb} Traf6^EP325 trbl^EP3519 trbl^EPg35131 Ubp64E^EP3187 w^+mC αTub84B^{Scer\UAS.P\T.T:Avic\GFP-S65C}
Constructs (8)
	P{-898/+133-bam:GFP} P{GAL4::VP16-nos.UTR} P{UAS-dpp.S} P{UAS-GFP.U} P{UAS-heph.S} P{UASp-bam.GFP} P{UASp-GFPS65C-αTub84B} P{UAS-tkv.Q253D.Nb}
Genes (89)
	arm Avic\GFP bam beag bun CG3308 CG5376 CG5807 CG8121 CG8833 CG10164 CG15161 CG17646 CG31176 CG33201 CG42336 corto Csp CycH dally dpn dpp Drsl1 Drsl6 EP303 EP416 EP569 EP605 EP639 EP917 EP1388 EP1457 EP1547 EP2031 EP2182 EP2522 EP3015a EP3269 EP3281 EP3289 EP3367 EP3582 EP3645 EP3683 Ets97D fabp Fas3 gbb GIIIspla2 gish glec Grip GstS1 hang heph His3 l(2)06655 l(3)L4092 lama Lmpt Lpin mam mav Men myo Nrx-IV numb Pdk1 RpL13A sax Scer\GAL4 scw shg SIFR SNF4Aγ Spec2 spin sqd T:Avic\GFP T:Hsim\VP16 tkv Traf6 trbl ttm2 Ubp64E w wun2 α-Spec αTub84B
Insertions (57)
	P{EP}bam^EP667 P{EP}beag^EP3260 P{EP}bun^EP2622 P{EP}CG2003^EP3204 P{EP}CG3308^EP1230 P{EP}CG5376^EP709 P{EP}CG5807^EP3455 P{EP}CG8833^EP3365 P{EP}CG17646^EP2506 P{EP}CG31176^EP3662 P{EP}corto^EP775 P{EP}Csp^EP659 P{EP}CycH^EP3658 P{EP}dally^EP581 P{EP}dpp^EP2232 P{EP}EP303^EP303 P{EP}EP416^EP416 P{EP}EP569^EP569 P{EP}EP605^EP605 P{EP}EP639^EP639 P{EP}EP917^EP917 P{EP}EP1388^EP1388 P{EP}EP1457^EP1457 P{EP}EP1547^EP1547 P{EP}EP2031^EP2031 P{EP}EP2182^EP2182 P{EP}EP2522^EP2522 P{EP}EP3015a^EP3015a P{EP}EP3269^EP3269 P{EP}EP3281^EP3281 P{EP}EP3367^EP3367 P{EP}EP3582^EP3582 P{EP}EP3645^EP3645 P{EP}EP3683^EP3683 P{EP}fabp^EP3252 P{EP}GIIIspla2^EP325 P{EP}gish^EP3652 P{EP}glec^EP410 P{EP}l(3)L4092^EP3690 P{EP}lama^EP3629 P{EP}Lmpt^EP610 P{EP}Lpin^EP2431 P{EP}mam^EP2198 P{EP}Men^EP500 P{EP}Nrx-IV^EP809 P{EP}numb^EP2505 P{EP}Pdk1^EP3091 P{EP}sda^EP3289 P{EP}SNF4Aγ^EP648 P{EP}SNF4Aγ^EP3015b P{EP}Spec2^EP3740 P{EP}spin^EP2363 P{EP}sqd^EP746 P{EP}trbl^EP3519 P{EP}Ubp64E^EP3187 P{EPg}trbl^EPg35131 P{GawB}ptc^559.1