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Reference
Citation	Shcherbata, H.R., Althauser, C., Findley, S.D., Ruohola-Baker, H. (2004). The mitotic-to-endocycle switch in Drosophila follicle cells is executed by Notch-dependent regulation of G1/S, G2/M and M/G1 cell-cycle transitions. Development 131(13): 3169--3181.
FlyBase ID	FBrf0179442
Type of publication	Research paper
Offprint Available	Yes
External Crossreferences
PubMed ID	15175253
PubMed Abstract	The Notch signaling pathway controls the follicle cell mitotic-to-endocycle transition in Drosophila oogenesis by stopping the mitotic cycle and promoting the endocycle. To understand how the Notch pathway coordinates this process, we have identified and performed a functional analysis of genes whose transcription is responsive to the Notch pathway at this transition. These genes include the G2/M regulator Cdc25 phosphatase, String; a regulator of the APC ubiquitination complex Hec/CdhFzr and an inhibitor of the CyclinE/CDK complex, Dacapo. Notch activity leads to downregulation of String and Dacapo, and activation of Fzr. All three genes are independently responsive to Notch. In addition, CdhFzr, an essential gene for endocycles, is sufficient to stop mitotic cycle and promote precocious endocycles when expressed prematurely during mitotic stages. In contrast, overexpression of the growth controller Myc does not induce premature endocycles but accelerates the kinetics of normal endocycles. We also show that Archipelago (Ago), a SCF-regulator is dispensable for mitosis, but crucial for endocycle progression in follicle epithelium. The results support a model in which Notch activity executes the mitotic-to-endocycle switch by regulating all three major cell cycle transitions. Repression of String blocks the M-phase, activation of Fzr allows G1 progression and repression of Dacapo assures entry into the S-phase. This study provides a comprehensive picture of the logic that external signaling pathways may use to control cell cycle transitions by the coordinated regulation of the cell cycle.
Biosis	2004.382120
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Language of publication	English
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Abbreviation	Development
Title	Development
Authors
Volume range	99-
Year range	1987-
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Place of publication	Cambridge
Language of publication	English
ISBN/ISSN	0950-1991
CODEN	DEVPED
Data from Reference
Alleles (30)
	CycA^Scer\UAS.cWa CycD^Scer\UAS.cDa CycE^Scer\UAS.cLa Dl^RevF10 Ecol\lacZ^stg.β-E6.4 Rbf^120a Rbf¹⁴ Scer\GAL4^Act5C.PP Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Su(H)⁸ Su(H)^del47 ago¹ ago³ ago⁴ ago^unspecified dap⁴ dap^{5gm.T:Hsap\MYC} dap^{Scer\UAS.cdNa} dm^Scer\UAS.cZa inx2^Scer\UAS.cSa mew^Δ.Scer\UAS p53^11-1B-1 p53^259H.GUS p53^5A-1-4 p53^Ct.GUS p53^GUS.PB rap^G0326 rap^Scer\UAS.cSa rap^ie28 stg^Scer\UAS.cNa
Constructs (16)
	P{GAL4-Act5C(-FRT).P} P{GAL4-Act5C(FRT.CD2).P} P{GUS-p53.259H} P{GUS-p53.Ct} P{GUS-p53} P{UAS-CycA.W} P{UAS-CycD.D} P{UAS-CycE.L} P{UAS-dap.dN} P{UAS-dm.Z} P{UAS-fzr.S} P{UAS-inx2.S} P{UAS-mew.Δ} P{UAS-stg.N} P{dap.5gm} P{stg-lacZ.β-E6.4}
Genes (22)
	CycA CycB CycD CycE Dl Ecol\lacZ Fas3 N Rbf Scer\GAL4 Su(H) T:Hsap\MYC Trf2 ago brc dap dm inx2 mew p53 rap stg
Insertions (11)
	P{GUS-p53.259H}3.1 P{GUS-p53.Ct}3.1 P{GUS-p53}2.1 P{UAS-dap.dN}II.2 P{UAS-dap.dN}II.3 P{UAS-dm.Z}132 P{UAS-fzr.S}II.1 P{UAS-fzr.S}III.2 P{UAS-stg.N}16 P{UAS-stg.N}4 P{lacW}rap^G0326