Reference Report

Reference
Citation	Sprecher, S.G., Mueller, M., Kammermeier, L., Miller, D.F.B., Kaufman, T.C., Reichert, H., Hirth, F. (2004). Hox gene cross-regulatory interactions in the embryonic brain of Drosophila. Mech. Dev. 121(6): 527--536. (Export to RIS)
FlyBase ID	FBrf0179451
Publication Type	Research paper
PubMed ID	15172684
PubMed Abstract	During embryonic development of the Drosophila brain, the Hox gene labial is required for the regionalized specification of the tritocerebral neuromere. In order to gain further insight into the mechanisms of Hox gene action in the CNS, we have studied the molecular and genetic basis of cross-regulatory interactions between labial and other more posterior Hox genes using the GAL4/UAS system for targeted misexpression. Misexpression of posterior Hox genes in the embryonic neuroectoderm results in a labial loss-of function phenotype and a corresponding lack of Labial protein expression in the tritocerebrum. This is due to repression of labial gene transcription in the embryonic brain. Enhancer analysis suggests that this transcriptional repression operates on a 3.65 kb brain-specific labial-enhancer element. A functional analysis of Antennapedia and Ultrabithorax protein domains shows that the transcriptional repression of labial requires homeodomain-DNA interactions but is not dependent on a functional hexapeptide. The repressive activity of a Hox protein on labial expression in the tritocerebrum can, however, be abolished by concomitant misexpression of a Hox protein and the cofactors Homothorax and nuclear-targeted Extradenticle. Taken together, these results provide novel and detailed insight into the cross-regulatory interactions of Hox genes in embryonic brain development and suggest that specification of tritocerebral neuronal identity requires equilibrated levels of a Hox protein and Hth and n-Exd cofactors.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Mech. Dev.
Title	Mechanisms of Development
Publication Year	1990-
ISBN/ISSN	0925-4773
Data from Reference
Alleles (20)
	abd-A^Scer\UAS.cGa Abd-B^Scer\UAS.cCa Antp^{A50,51.Scer\UAS} Antp^K50.Scer\UAS Antp^Scer\UAS.cMb Antp^{ΔHD.Scer\UAS} Dfd^Scer\UAS.cBa Ecol\lacZ^lab.3.65 exd^{Scer\UAS.NLS.T:Zzzz\FLAG} hth^Scer\UAS.cPa lab¹⁴ lab^Scer\UAS.cMa pb^Scer\UAS.A Scer\GAL4^elav-C155 Scer\GAL4^insc-Mz1407 Scer\GAL4^sca-537.4 Scr^Scer\UAS.cMa Ubx^Scer\UAS.cCa Ubx^Scer\UAS.cMa Ubx^{YAAA.Scer\UAS}
Constructs (16)
	P{3.65lab66a} P{UAS-abd-A.G} P{UAS-Abd-B.m.C} P{UAS-Antp.A50,51} P{UAS-Antp.Mb} P{UAS-Antp.Q50K.C} P{UAS-AntpΔHD} P{UAS-Dfd.B} P{UAS-hth.P} P{UAS-lab.M} P{UAS-NLS-exd} P{UAS-pb.A} P{UAS-Scr.M} P{UAS-Ubx.Ia.C} P{UAS-Ubx.M} P{UAS-Ubx.YAAA}
Genes (16)
	abd-A Abd-B Antp Dfd Ecol\lacZ elav exd Fas2 hth lab pb repo Scer\GAL4 Scr T:Zzzz\FLAG Ubx
Insertions (3)
	P{GawB}elav^C155 P{GawB}insc^Mz1407 P{GawB}sca^537.4