Reference Report

Reference
Citation	Sutrias-Grau, M., Arnosti, D.N. (2004). CtBP contributes quantitatively to Knirps repression activity in an NAD binding-dependent manner. Mol. Cell. Biol. 24(13): 5953--5966. (Export to RIS)
FlyBase ID	FBrf0179465
Publication Type	Research paper
PubMed ID	15199149
PubMed Abstract	Transcriptional repressors often employ multiple activities, but the molecular mechanisms and physiological relevance of this functional diversity remain obscure. The Drosophila melanogaster Knirps repressor uses CtBP corepressor-dependent and -independent pathways. To separately analyze the components of Knirps repression activity, we elucidated the specific repression properties of CtBP and of Knirps subdomains. Like Knirps, CtBP represses adjacent transcriptional activators; but unlike Knirps, CtBP is unable to repress basal promoter elements. We determined that the ability of CtBP to recapitulate only a subset of Knirps activities is due to a quantitative, rather than qualitative, deficiency in repression activity. The CtBP-dependent portion of Knirps synergizes with the CtBP-independent repression activity to potently repress promoter elements from enhancer- or promoter-proximal positions. This result indicates that multiple repression activities are combined to exceed critical thresholds on target genes. CtBP mutant proteins unable to bind NAD fail to interact with DNA-bound factors. We show that DNA-binding Gal4-CtBP fusion proteins also require NAD binding for activity, indicating that NAD plays a role in repression at a step subsequent to CtBP recruitment to the promoter.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Alleles (25)
	CtBP^{L.GA.Kr.T:Scer\GAL4,T:Zzzz\FLAG} CtBP^{L.HQ.Kr.T:Scer\GAL4,T:Zzzz\FLAG} CtBP^{L.Kr.T:Scer\GAL4,T:Zzzz\FLAG} CtBP^{S.Kr.T:Scer\GAL4,T:Zzzz\FLAG} Ecol\lacZ^{eve.st3.rho.Scer\UAS} Ecol\lacZ^gt.55x2 Ecol\lacZ^{rho.330.twi.2xPE.2x55.kni} Ecol\lacZ^{rho.twi.2xPE.2x55.Scer\UAS} Ecol\lacZ^{rho.twi.2xPE.4x55.Scer\UAS} Ecol\lacZ^{rho.twi.2xPE.6x55.Scer\UAS} Ecol\lacZ^{rho.twi.2xPE.twi.dl.2x55.Scer\UAS} Ecol\lacZ^{twi.dl.2x55.Scer\UAS} Ecol\lacZ^{twi.dl.2x130.Scer\UAS} Ecol\lacZ^{twi.dl.55.2x55.Scer\UAS} Ecol\lacZ^{twi.dl.55.2x130.Scer\UAS} Ecol\lacZ^{twi.dl.110.2x55.Scer\UAS} Ecol\lacZ^{twi.dl.110.2x130.Scer\UAS} Ecol\lacZ^{twi.dl.165.2x55.Scer\UAS} Ecol\lacZ^{twi.dl.165.2x130.Scer\UAS} kni^{75-330.Kr.T:Scer\GAL4,T:Zzzz\FLAG} kni^{75-429.Kr.T:Scer\GAL4,T:Zzzz\FLAG} kni^{75-429.ΔPMDL.Kr.T:Scer\GAL4,T:Zzzz\FLAG} kni^{139-330.Kr.T:Scer\GAL4,T:Zzzz\FLAG} kni^{189-330.Kr.T:Scer\GAL4,T:Zzzz\FLAG} kni^{202-358.Kr.T:Scer\GAL4,T:Zzzz\FLAG}
Constructs (24)
	P{CRT4U(-55)-lacZ} P{CRT6U(-55)-lacZ} P{CRTtdU(-55)-lacZ} P{CRTU(-55)-lacZ} P{Ctd(55)U(-55)-lacZ} P{Ctd(55)U(-130)-lacZ} P{Ctd(110)U(-55)-lacZ} P{Ctd(110)U(-130)-lacZ} P{Ctd(165)U(-55)-lacZ} P{Ctd(165)U(-130)-lacZ} P{CtdU(-55)-lacZ} P{CtdU(-130)-lacZ} P{Kr-Gal4-CtBP.L.GA} P{Kr-Gal4-CtBP.L.HQ} P{Kr-Gal4-CtBP.L} P{Kr-Gal4-CtBP.S} P{Kr-GAL4-kni.75-330} P{Kr-GAL4-kni.75-429.ΔPMDL} P{Kr-GAL4-kni.75-429} P{Kr-GAL4-kni.139-330} P{Kr-GAL4-kni.189-330} P{Kr-GAL4-kni.202-358} P{lacZ^{eve.st3.rho.UAS}} P{rho330-2xtwiPE-2xknirps-lacZ}
Genes (5)
	CtBP Ecol\lacZ kni T:Scer\GAL4 T:Zzzz\FLAG
Natural transposons (1)
	P-element