Reference Report

Reference
Citation	Domingos, P.M., Broan, S., Barrio, R., Ratnakumar, K., Frankfort, B.J., Mardon, G., Steller, H., Mollereau, B. (2004). Regulation of R7 and R8 differentiation by the spalt genes. Dev. Biol. 273(1): 121--133. (Export to RIS)
FlyBase ID	FBrf0179729
Publication Type	Research paper
PubMed ID	15302602
PubMed Abstract	Photoreceptor development begins in the larval eye imaginal disc, where eight distinct photoreceptor cells (R1-R8) are sequentially recruited into each of the developing ommatidial clusters. Final photoreceptor differentiation, including rhabdomere formation and rhodopsin expression, is completed during pupal life. During pupation, spalt was previously proposed to promote R7 and R8 terminal differentiation. Here we show that spalt is required for proper R7 differentiation during the third instar larval stage since the expression of several R7 larval markers (prospero, enhancer of split mdelta0.5, and runt) is lost in spalt mutant clones. In R8, spalt is not required for cell specification or differentiation in the larval disc but promotes terminal differentiation during pupation. We show that spalt is necessary for senseless expression in R8 and sufficient to induce ectopic senseless in R1-R6 during pupation. Moreover, misexpression of spalt or senseless is sufficient to induce ectopic rhodopsin 6 expression and partial suppression of rhodopsin 1. We demonstrate that spalt and senseless are part of a genetic network, which regulates rhodopsin 6 and rhodopsin 1. Taken together, our results suggest that while spalt is required for R7 differentiation during larval stages, spalt and senseless promote terminal R8 differentiation during pupal stages, including the regulation of rhodopsin expression.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (1)
	Df(2L)32FP-5
Alleles (11)
	Avic\GFP^Ubi-p63E.PD Ecol\lacZ^HLHmδ.0.5 Ecol\lacZ^klg-H214 Ecol\lacZ^Rh3.-247+18 salm^Scer\UAS.cKa Scer\GAL4^ninaE.PT Scer\GAL4^salr.Ola.cDa sens^E1 sens^Scer\UAS.cNa svp^rA028 W^GMR.PG
Constructs (8)
	P{GMR-hid} P{HLHmδ-lacZ.0.5} P{Ola-GAL4} P{rh1-GAL4} P{Rh3-lacZ.-247+18} P{UAS-salm.K} P{UAS-sens.N} P{Ubi-GFP.D}
Genes (20)
	Avic\GFP B-H1 boss E(spl)mδ-HLH Ecol\lacZ elav futsch klg ninaE pros Rh3 Rh4 Rh5 Rh6 run salm Scer\GAL4 sens svp W
Insertions (5)
	P{HZ}klg^H214 P{PZ}svp^rA028 P{UAS-salm.K}RS90 P{UAS-sens.N}c5c P{Ubi-GFP.D}61EF