Subject: DrosDel deletions confirmed by PCR and genetic tests.
Hi Rachel,
The following DrosDel deletions have been confirmed by PCR (using the
3-step process described on the web site) and by genetic
complementation (see following mail from Roote) against known mutations
on the Release3.1 sequence.
Df(2L)ED3
Df(2R)ED1552
Df(2R)ED1
Df(3L)ED4177
Df(3R)ED5020
Df(3R)ED5021
Df(3R)ED5071
Df(3R)ED5095
Df(3R)ED5100
Df(3R)ED5138
Df(3R)ED5142
Df(3R)ED5147
Df(3R)ED5156
Df(3R)ED5177
Df(3R)ED5196
Df(3R)ED5223
Df(3R)ED5230
Df(3R)ED5296
Df(3R)ED5429
Df(3R)ED5516
Df(3R)ED5558
Df(3R)ED5559
Df(3R)ED5608
Df(3R)ED5622
Df(3R)ED5642
Df(3R)ED5705
Df(3R)ED5780
Df(3R)ED2
Df(3R)ED5911
Df(3R)ED5938
Df(3R)ED6025
Df(3R)ED6076
Df(3R)ED6085
Df(3R)ED6093
Df(3R)ED6096
Df(3R)ED6103
Df(3R)ED6187
Df(3R)ED6220
Df(3R)ED6232
Df(3R)ED6235
Df(3R)ED6242
Df(3R)ED6255
Df(3R)ED6310
Df(3R)ED6316
Df(4)ED6364
Df(4)ED6366
Df(4)ED6382
Df(4)ED6384
Df(1)ED7374
Cheers,
Ed
\--
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Dr. Ed Ryder
Department of Genetics, University of Cambridge,
=====================================
Subject: ED genetic confirmation
Rachel,
Complementation crosses with EDs.
Df(2R)ED1552 (from Ed's list) not confirmed genetically.
John
'Df name','non-complementing','complementing (viable, no obvious phenotype)'
'Df(2L)ED3','osp, noc (weak), gft, nht','el, esg'
'Df(2R)ED1','RhoGEF2, Df(2R)P803-D15','l(2)k10815, Gst2, l(2)BG00665,
l(2)k08805'
'Df(3L)ED4177','emc',
'Df(3R)ED5020','hkb, tub',
'Df(3R)ED5021','hkb, tub',
'Df(3R)ED5071','opa, Karybeta3, tub',
'Df(3R)ED5095','Karybeta3, opa',
'Df(3R)ED5100','Karybeta3, tub, corto07128b (semi-lethal)',
'Df(3R)ED5138','opa, corto',
'Df(3R)ED5142','opa, corto, Karybeta3',
'Df(3R)ED5147','cno, Hph',
'Df(3R)ED5156','Hph, kkv','cno'
'Df(3R)ED5177','Atu','Rga03834 (reported by Sprad to be a semi-lethal).
elF-5C (ED5177 predicted to delete only 540bp of gene)'
'Df(3R)ED5196','cas',
'Df(3R)ED5223','dsx, Tom34',
'Df(3R)ED5230','Tom34, stck',
'Df(3R)ED5296','Mcm2',
'Df(3R)ED5429','Scm, dmt',
'Df(3R)ED5516','pros','RpL3KG05440'
'Df(3R)ED5558','aur, svp, sad',
'Df(3R)ED5559','aur, sad',
'Df(3R)ED5608','Df(3R)Exel7318','Df(3R)kar-H5'
'Df(3R)ED5622','ems, flflL4179 (unverified lethal: over ED5622 wings
held-down, veins disrupted, low female fertility.)',
'Df(3R)ED5642','trx, ems, flflL4179 (unverified lethal: over ED5622 wings
held-down, veins disrupted, low female fertility.)',
'Df(3R)ED5705','Atx2',
'Df(3R)ED5780','Df(3R)ED5794','Dadj1e4 and Sur-8e02803, unverified lethals'
'Df(3R)ED2','fray07551, Df(3R)Cha-9, l(3)S007302 (unverified lethal),
Df(3R)Dl-M2','l(3)02515 and l(3)S148213 unverified lethals, gl'
'Df(3R)ED5911','cdi, sqz, nos',
'Df(3R)ED5938','sqz',
'Df(3R)ED6025','GluCalpha, bnl',
'Df(3R)ED6076','tsl, E2f, how',
'Df(3R)ED6085','how, sar1, tld',
'Df(3R)ED6093','sar1, Dph5',
'Df(3R)ED6096','hh, cnc, Df(3R)ED6091',
'Df(3R)ED6103','hh, cnc',
'Df(3R)ED6187','Apc2, crib',
'Df(3R)ED6220','CG11836f02631 (unverified lethal), tld',
'Df(3R)ED6232','gro',
'Df(3R)ED6235','scrib','gro'
'Df(3R)ED6242','pll',
'Df(3R)ED6255','pll',
'Df(3R)ED6310','stg','Ef1gammae00972 (unverified lethal)'
'Df(3R)ED6316','kay, Dr, stg, Df(3R)ED6310',
'Df(4)ED6364','RpS3A (note: stock is phenotypically Minute), panciD, gvl','bt'
'Df(4)ED6366','panciD, gvl','bt'
'Df(4)ED6382','lgs, eyD, bt','pan, ci, gvl'
'Df(4)ED6384','eyD, bt','ci, gvl'
'Df(1)ED7374','if',