Reference Report

Reference
Citation	Pastor-Pareja, J.C., Grawe, F., Martin-Blanco, E., Garcia-Bellido, A. (2004). Invasive cell behavior during Drosophila imaginal disc eversion is mediated by the JNK signaling cascade. Dev. Cell 7(3): 387--399. (Export to RIS)
FlyBase ID	FBrf0180122
Publication Type	Research paper
PubMed ID	15363413
PubMed Abstract	Drosophila imaginal discs are monolayered epithelial invaginations that grow during larval stages and evert at metamorphosis to assemble the adult exoskeleton. They consist of columnar cells, forming the imaginal epithelium, as well as squamous cells, which constitute the peripodial epithelium and stalk (PS). Here, we uncover a new morphogenetic/cellular mechanism for disc eversion. We show that imaginal discs evert by apposing their peripodial side to the larval epidermis and through the invasion of the larval epidermis by PS cells, which undergo a pseudo-epithelial-mesenchymal transition (PEMT). As a consequence, the PS/larval bilayer is perforated and the imaginal epithelia protrude, a process reminiscent of other developmental events, such as epithelial perforation in chordates. When eversion is completed, PS cells localize to the leading front, heading disc expansion. We found that the JNK pathway is necessary for PS/larval cells apposition, the PEMT, and the motile activity of leading front cells.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (1)
	Df(3R)p712
Alleles (31)
	Avic\GFP^{S65T.Scer\UAS} hep^r75 puc^B48R23 puc^E69 puc^E69-A puc^E69-C puc^E69-E puc^E69-F puc^E69-G puc^E69-H puc^E69-I puc^E69-K puc^E69-M puc^E69-Q puc^E69-S puc^Scer\UAS.cMa Scer\GAL4^Act5C.PI Scer\GAL4^puc-E69-A Scer\GAL4^puc-E69-C Scer\GAL4^puc-E69-E Scer\GAL4^puc-E69-F Scer\GAL4^puc-E69-G Scer\GAL4^puc-E69-H Scer\GAL4^puc-E69-I Scer\GAL4^puc-E69-K Scer\GAL4^puc-E69-M Scer\GAL4^puc-E69-Q Scer\GAL4^puc-E69-S Scer\GAL4^{Scer\FRT.Act5C} shg^{Ubi-p63E.T:Avic\GFP-rs} y^Scer\UAS.cCa
Constructs (7)
	P{Act5C-GAL4} P{AyGAL4} P{GawB} P{UAS-GFP.S65T} P{UAS-puc.M} P{UAS-y.C} P{Ubi-p63E-shg.GFP}
Genes (7)
	arm Avic\GFP hep puc Scer\GAL4 shg y
Insertions (12)
	P{A92}puc^E69 P{GawB}puc^E69-A P{GawB}puc^E69-C P{GawB}puc^E69-E P{GawB}puc^E69-F P{GawB}puc^E69-G P{GawB}puc^E69-H P{GawB}puc^E69-I P{GawB}puc^E69-K P{GawB}puc^E69-M P{GawB}puc^E69-Q P{GawB}puc^E69-S