Reference Report

Reference
Citation	Brodu, V., Elstob, P.R., Gould, A.P. (2004). EGF receptor signaling regulates pulses of cell delamination from the Drosophila ectoderm. Dev. Cell 7(6): 885--895. (Export to RIS)
FlyBase ID	FBrf0180129
Publication Type	Research paper
PubMed ID	15572130
PubMed Abstract	Many different intercellular signaling pathways are known but, for most, it is unclear whether they can generate oscillating cell behaviors. Here we use time-lapse analysis of Drosophila embryogenesis to show that oenocytes delaminate from the ectoderm in discrete bursts of three. This pulsatile process has a 1 hour period, occurs without cell division, and requires a localized EGF receptor (EGFR) response. High-threshold EGFR targets are sequentially activated in rings of three cells, prefiguring the temporal pattern of delamination. Surprisingly, widespread misexpression of the relevant activating ligand, Spitz, is compatible with robust delamination pulses. Moreover, although Spitz ligand becomes limiting after only two pulses, artificially prolonging its secretion generates up to six additional cycles, revealing a rhythmic underlying mechanism. These findings illustrate how intercellular signaling and cell movements can generate multiple cycles of a cell behavior, despite individual cells experiencing only one cycle of receptor activation.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (1)
	Df(3R)p13
Alleles (27)
	abd-A^M1 aop^yan-1 aos^Scer\UAS.cHa aos^Δ7 ato¹ Avic\GFP^{S65T.Scer\UAS.T:SV40\nls2} Dl^9P Dl^D.Scer\UAS Ecol\lacZ^{Scer\UAS.T:SV40\nls2} Ecol\lacZ^svp-don1 Ecol\lacZ^{svp-lacZ.Δ18} Egfr^{act.B.Scer\UAS} Egfr^f2 N^55e11 N^ICN.Scer\UAS O-fut1^{dsRNA.Scer\UAS} O-fut1^Scer\UAS.cOa pnt^P1.Scer\UAS Ras85D^V12.Scer\UAS rho^{Scer\UAS.cdCa} Scer\GAL4^en-e16E Scer\GAL4^{F442A.αTub84B.T:Hsim\VP16} Scer\GAL4^salm-459.2 Ser^RX106 spi^s.Scer\UAS svp^don1 svp^lacZ.Δ18
Constructs (14)
	P{?A92} P{GAL4-αTub84B(-FRT).VP16^F442A} P{UAS-Dl.DN} P{UAS-Egfr.act.B} P{UAS-GFP^S65T.nls} P{UAS-gil.H} P{UAS-lacZ.NZ} P{UAS-N.ICN} P{UAS-O-fut1.dsRNA} P{UAS-O-fut1.O} P{UAS-pnt.P1} P{UAS-Ras85D.V12} P{UAS-spi.s} P{UAS-ve.dC}
Genes (22)
	abd-A aop aos ato Avic\GFP Cyp4g1 Dl Ecol\lacZ Egfr en N O-fut1 peb pnt Ras85D rho rl Scer\GAL4 Ser spi svp vvl
Insertions (4)
	P{?A92}svp^lacZ.Δ18 P{A92}svp^don1 P{en2.4-GAL4}e16E P{GawB}459.2