Reference Report

Reference
Citation	Apitz, H., Kambacheld, M., Hoehne, M., Ramos, R.G.P., Straube, A., Fischbach, K.F. (2004). Identification of regulatory modules mediating specific expression of the roughest gene in Drosophila melanogaster. Dev. Genes Evol. 214(9): 453--459.
FlyBase ID	FBrf0180136
Type of publication	Research paper
Offprint Available	No
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PubMed ID	15278452
PubMed Abstract	Roughest (Rst) is a cell adhesion molecule of the immunoglobulin superfamily with pleiotropic functions during the development of Drosophila melanogaster. It has been shown to be involved in cell sorting before apoptosis in the developing compound eye, in fusion processes of embryonic muscle development and in axonal pathfinding. In accordance with its multiple functions, the rst gene shows a dynamic expression pattern throughout the development of Drosophila. In order to understand the transcriptional regulation of rst expression we have identified rst cis regulatory sequences in an enhancer detection screen. By dissection of the identified rst cis regulatory sequences we identified several distinct rst regulatory modules. Among others these include elements for expression in interommatidial cells of the pupal eye disc at a time when apoptotic decisions are made in these cells and elements for expression in the embryonic mesoderm. The expression of rst in the embryonic mesoderm is regulated by at least two separate modules.
Biosis	2004.446344
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Language of publication	English
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Abbreviation	Dev. Genes Evol.
Title	Development genes and evolution
Authors
Volume range	206-
Year range	1996-
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Place of publication	Berlin
Language of publication	English
ISBN/ISSN	0949-944X
CODEN
Data from Reference
Alleles (13)
	Ecol\lacZ^Scer\UAS.cBa Ecol\lacZ^rstF1 Ecol\lacZ^rstF2 Ecol\lacZ^rstF3 Ecol\lacZ^rstF4 Ecol\lacZ^rstF5 Ecol\lacZ^rstF6 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^rstF5 Scer\GAL4^rstF5p Scer\GAL4^rstF6 Scer\GAL4^rstF6d Scer\GAL4^rstF6p
Constructs (13)
	P{UAS-lacZ.B} P{UAS-mCD8::GFP.L} P{rst.F1-lacZ} P{rst.F2-lacZ} P{rst.F3-lacZ} P{rst.F4-lacZ} P{rst.F5-GAL4} P{rst.F5-lacZ} P{rst.F5p-GAL4} P{rst.F6-GAL4} P{rst.F6-lacZ} P{rst.F6d-GAL4} P{rst.F6p-GAL4}
Genes (5)
	Ecol\lacZ Mmus\Cd8a Scer\GAL4 T:Avic\GFP rst
Insertions (2)
	P{UAS-lacZ.B}Bg4-2-4b P{UAS-lacZ.B}melt^Bg4-1-2