Reference Report

Reference
Citation	Whited, J.L., Cassell, A., Brouillette, M., Garrity, P.A. (2004). Dynactin is required to maintain nuclear position within postmitotic Drosophila photoreceptor neurons. Development 131(19): 4677--4686. (Export to RIS)
FlyBase ID	FBrf0180149
Publication Type	Research paper
PubMed ID	15329347
PubMed Abstract	How a nucleus is positioned within a highly polarized postmitotic animal cell is not well understood. In this work, we demonstrate that the Dynactin complex (a regulator of the microtubule motor protein Dynein) is required to maintain the position of the nucleus within post-mitotic Drosophila melanogaster photoreceptor neurons. We show that multiple independent disruptions of Dynactin function cause a relocation of the photoreceptor nucleus toward the brain, and that inhibiting Dynactin causes the photoreceptor to acquire a bipolar appearance with long leading and trailing processes. We find that while the minus-end directed motor Dynein cooperates with Dynactin in positioning the photoreceptor nucleus, the plus-end directed microtubule motor Kinesin acts antagonistically to Dynactin. These data suggest that the maintenance of photoreceptor nuclear position depends on a balance of plus-end and minus-end directed microtubule motor function.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(2L)E.2
Alleles (16)
	cpb^+t4.5 cpb^F44 cpb^M143 cpb^Scer\UAS.cWa Dmn^{Scer\UAS.T:Avic\GFP} Ecol\lacZ^GMR.C.hs Gl¹ Gl^Δ.Scer\UAS Khc::nod^{Scer\UAS.T:Ecol\lacZ} Khc⁸ Khc^k13219 Khc^k13314 Scer\GAL4^elav.PLu Scer\GAL4^Glass38-1 Scer\GAL4^αTub84B.PL sw^Dic-2
Constructs (8)
	P{cpb^+t4.5} P{GAL4-elav.L} P{Glass38.1-GAL4} P{GMR-lacZ.C(38.1)} P{tubP-GAL4} P{UAS-cpb.W} P{UAS-Gl^Δ} P{UAS-Khc::nod::lacZ}
Genes (14)
	arm chp cpb Dmn Ecol\lacZ elav Gl Khc Khc::nod Patj Scer\GAL4 sw T:Avic\GFP T:Ecol\lacZ
Insertions (1)
	P{lacW}Khc^k13314