Reference Report

Reference
Citation	Tayler, T.D., Robichaux, M.B., Garrity, P.A. (2004). Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins. Development 131(23): 5935--5945. (Export to RIS)
FlyBase ID	FBrf0180181
Publication Type	Research paper
PubMed ID	15525663
PubMed Abstract	Brain morphogenesis depends on the maintenance of boundaries between populations of non-intermingling cells. We used molecular markers to characterize a boundary within the optic lobe of the Drosophila brain and found that Slit and the Robo family of receptors, well-known regulators of axon guidance and neuronal migration, inhibit the mixing of adjacent cell populations in the developing optic lobe. Our data suggest that Slit is needed in the lamina to prevent inappropriate invasion of Robo-expressing neurons from the lobula cortex. We show that Slit protein surrounds lamina glia, while the distal cell neurons in the lobula cortex express all three Drosophila Robos. We examine the function of these proteins in the visual system by isolating a novel allele of slit that preferentially disrupts visual system expression of Slit and by creating transgenic RNA interference flies to inhibit the function of each Drosophila Robo in a tissue-specific fashion. We find that loss of Slit or simultaneous knockdown of Robo, Robo2 and Robo3 causes distal cell neurons to invade the lamina, resulting in cell mixing across the lamina/lobula cortex boundary. This boundary disruption appears to lead to alterations in patterns of axon navigation in the visual system. We propose that Slit and Robo-family proteins act to maintain the distinct cellular composition of the lamina and the lobula cortex.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (25)
	Avic\GFP^GMR.PG Avic\GFP^Scer\UAS.cUa Ecol\lacZ^loco-3-109 Ecol\lacZ^repo-03702 Ecol\lacZ^{ro.T:Btau\MAPT} Ecol\lacZ^sli-05248 eya² lea^{dsRNA.Scer\UAS} lea^X123 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} robo3¹ robo3^{dsRNA.Scer\UAS} robo⁵ robo^{dsRNA.Scer\UAS} Scer\GAL4^bi-md653 Scer\GAL4^elav-C155 Scer\GAL4^GMR.PF Scer\GAL4^sca-375.1 Scer\GAL4^αTub84B.PL sli² sli⁰⁵²⁴⁸ sli^E-158 sli^k04807b sli^Scer\UAS.cKa w^+mC
Constructs (10)
	P{GAL4-ninaE.GMR} P{GMR-GFP.G} P{ro.lacZ-tau.G} P{tubP-GAL4} P{UAS-GFP.U} P{UAS-lea.RNAi} P{UAS-mCD8::GFP.L} P{UAS-robo3.RNAi} P{UAS-robo.RNAi} P{UAS-sli.K}
Genes (18)
	Avic\GFP chp Ecol\lacZ elav eya Fas3 lea loco Mmus\Cd8a repo robo robo3 sca Scer\GAL4 sli T:Avic\GFP T:Btau\MAPT w
Insertions (7)
	P{GAL4}375.1 P{GawB}bi^md653 P{GawB}elav^C155 P{lacW}sli^k04807b P{lacZ}loco^3-109 P{PZ}repo⁰³⁷⁰² P{PZ}sli⁰⁵²⁴⁸