Reference Report

Reference
Citation	Winter, S.E., Campbell, G. (2004). Repression of Dpp targets in the Drosophila wing by Brinker. Development 131(24): 6071--6081. (Export to RIS)
FlyBase ID	FBrf0180185
Publication Type	Research paper
PubMed ID	15537684
PubMed Abstract	Patterning along developing body axes is regulated by gradients of transcription factors, which activate or repress different genes above distinct thresholds. Understanding differential threshold responses requires knowledge of how these factors regulate transcription. In the Drosophila wing, expression of genes such as omb and sal along the anteroposterior axis is restricted by lateral-to-medial gradients of the transcriptional repressor Brinker (Brk). omb is less sensitive to repression by Brk than sal and is consequently expressed more laterally. Contrary to previous suggestions, we show that Brk cannot repress simply by competing with activators, but requires specific repression domains along with its DNA-binding domain. Brk possesses at least three repression domains, but these are not equivalent; one, 3R, is sufficient to repress omb but not sal. Thus, although sal and omb show quantitative differences in their response to Brk, there are qualitative differences in the mechanisms that Brk uses to repress them.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (40)
	Avic\GFP^{hs.T:Hsap\MYC} Avic\GFP^{S65T.Ubi-p63E.T:SV40\nls2} brk^{3M.Scer\UAS.T:Ivir\HA1} brk^{53.Scer\UAS.T:Ivir\HA1} brk^{A2.Scer\UAS.T:Ivir\HA1} brk^{A438-53.Scer\UAS} brk^{A438-C.Scer\UAS} brk^{A438-D44.Scer\UAS} brk^{A438-F2.Scer\UAS} brk^{A438-S4.Scer\UAS} brk^{A.Scer\UAS.T:Ivir\HA1} brk^{CM.Scer\UAS.T:Ivir\HA1} brk^{CMGM.Scer\UAS.T:Ivir\HA1} brk^E427 brk^{EC.Scer\UAS.T:Ivir\HA1,T:SV40\nls2} brk^{F124.Scer\UAS.T:Ivir\HA1} brk^F124 brk^F138 brk^{GM.Scer\UAS.T:Ivir\HA1} brk^M68 brk^{NA.Scer\UAS.T:Ivir\HA1} brk^{NLS.Scer\UAS.T:Ivir\HA1,T:SV40\nls2} brk^{NLSW.Scer\UAS.T:Ivir\HA1,T:SV40\nls2} brk^{Scer\FRT.Scer\UAS} brk^Scer\UAS.cWa brk^{Scer\UAS.T:Ivir\HA1} brk^{Stop1.Scer\UAS.T:Ivir\HA1} brk^{Stop1NA.Scer\UAS.T:Ivir\HA1} brk^{Stop1NAC.Scer\UAS.T:Ivir\HA1} brk^{Stop1ΔA17.Scer\UAS.T:Ivir\HA1} CtBP^87De-10 Ecol\lacZ^bi-Pol-1 Ecol\lacZ^Ubx.Bhz Ecol\lacZ^vg.806 gro^E48 Scer\GAL4^C-765 Scer\GAL4^en-e16E Scer\GAL4^how-24B w^+mC y^+t*
Constructs (28)
	P{Bhz} P{hsp70-Myc-GFP} P{UAS(FRT.y⁺)brk.A438} P{UAS(-FRT)brk.A438-53} P{UAS(-FRT)brk.A438} P{UAS(-FRT)brk.A438-C} P{UAS(-FRT)brk.A438-D44} P{UAS(-FRT)brk.A438-F2} P{UAS(-FRT)brk.A438-S4} P{UAS-brk.3M} P{UAS-brk.3PF3} P{UAS-brk.53} P{UAS-brk.A2} P{UAS-brk.A} P{UAS-brk.CM} P{UAS-brk.CMGM} P{UAS-brk.EC} P{UAS-brk.F124} P{UAS-brk.GM} P{UAS-brk.NA} P{UAS-brk.NLS} P{UAS-brk.NLSW} P{UAS-brk.Stop1} P{UAS-brk.Stop1NA} P{UAS-brk.Stop1NAC} P{UAS-brk.Stop1ΔA17} P{Ubi-GFP(S65T)nls} P{vg(806)-lacZ}
Genes (16)
	Avic\GFP bi brk CtBP Dpse\brk Ecol\lacZ gro salm Scer\GAL4 T:Hsap\MYC T:Ivir\HA1 T:SV40\nls2 Ubx vg w y
Insertions (4)
	P{en2.4-GAL4}e16E P{GawB}C-765 P{GawB}how^24B P{lacW}bi^Pol-1