Reference Report

Reference
Citation	Lundstrom, A., Gallio, M., Englund, C., Steneberg, P., Hemphala, J., Aspenstrom, P., Keleman, K., Falileeva, L., Dickson, B.J., Samakovlis, C. (2004). Vilse, a conserved Rac/Cdc42 GAP mediating Robo repulsion in tracheal cells and axons. Genes Dev. 18(17): 2161--2171. (Export to RIS)
FlyBase ID	FBrf0180254
Publication Type	Research paper
PubMed ID	15342493
PubMed Abstract	Slit proteins steer the migration of many cell types through their binding to Robo receptors, but how Robo controls cell motility is not clear. We describe the functional analysis of vilse, a Drosophila gene required for Robo repulsion in epithelial cells and axons. Vilse defines a conserved family of RhoGAPs (Rho GTPase-activating proteins), with representatives in flies and vertebrates. The phenotypes of vilse mutants resemble the tracheal and axonal phenotypes of Slit and Robo mutants at the CNS midline. Dosage-sensitive genetic interactions between vilse, slit, and robo mutants suggest that vilse is a component of robo signaling. Moreover, overexpression of Vilse in the trachea of robo mutants ameliorates the phenotypes of robo, indicating that Vilse acts downstream of Robo to mediate midline repulsion. Vilse and its human homolog bind directly to the intracellular domains of the corresponding Robo receptors and promote the hydrolysis of RacGTP and, less efficiently, of Cdc42GTP. These results together with genetic interaction experiments with robo, vilse, and rac mutants suggest a mechanism whereby Robo repulsion is mediated by the localized inactivation of Rac through Vilse.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (1)
	Df(3R)e-N12
Alleles (14)
	Cdc42³ Ecol\lacZ^trh-1-eve-1 lea^unspecified Rac1^J10 Rac1^J11 Rac2^Δ RhoGAP93B¹ RhoGAP93B^S138901a RhoGAP93B^Scer\UAS.cLa robo¹ robo⁴ Scer\GAL4^bs-23.26 Scer\GAL4^btl.PS sli²
Constructs (3)
	P{GAL4-bs.23.26} P{GAL4-btl.S} P{UAS-vilse.L}
Genes (11)
	bs Cdc42 Ecol\lacZ Fas2 lea Rac1 Rac2 RhoGAP93B robo Scer\GAL4 sli
Insertions (3)
	P{ET-L}trh-1-eve-1 P{lacW}RhoGAP93B^S138901a P{lacW}S138901a