Reference Report

Reference
Citation	Wilk, R., Pickup, A.T., Hamilton, J.K., Reed, B.H., Lipshitz, H.D. (2004). Dose-sensitive autosomal modifiers identify candidate genes for tissue autonomous and tissue nonautonomous regulation by the Drosophila nuclear zinc-finger protein, hindsight. Genetics 168(1): 281--300. (Export to RIS)
FlyBase ID	FBrf0180294
Publication Type	Research paper
PubMed ID	15454543
PubMed Abstract	The nuclear zinc-finger protein encoded by the hindsight (hnt) locus regulates several cellular processes in Drosophila epithelia, including the Jun N-terminal kinase (JNK) signaling pathway and actin polymerization. Defects in these molecular pathways may underlie the abnormal cellular interactions, loss of epithelial integrity, and apoptosis that occurs in hnt mutants, in turn causing failure of morphogenetic processes such as germ band retraction and dorsal closure in the embryo. To define the genetic pathways regulated by hnt, 124 deficiencies on the second and third chromosomes and 14 duplications on the second chromosome were assayed for dose-sensitive modification of a temperature-sensitive rough eye phenotype caused by the viable allele, hntpeb; 29 interacting regions were identified. Subsequently, 438 P-element-induced lethal mutations mapping to these regions and 12 candidate genes were tested for genetic interaction, leading to identification of 63 dominant modifier loci. A subset of the identified mutants also dominantly modify hnt308-induced embryonic lethality and thus represent general rather than tissue-specific interactors. General interactors include loci encoding transcription factors, actin-binding proteins, signal transduction proteins, and components of the extracellular matrix. Expression of several interactors was assessed in hnt mutant tissue. Five genes--apontic (apt), Delta (Dl), decapentaplegic (dpp), karst (kst), and puckered (puc)--are regulated tissue autonomously and, thus, may be direct transcriptional targets of HNT. Three of these genes--apt, Dl, and dpp--are also regulated nonautonomously in adjacent non-HNT-expressing tissues. The expression of several additional interactors--viking (vkg), Cg25, and laminin-alpha (LanA)-is affected only in a nonautonomous manner.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (29)
	Df(2L)Dwee-δ5 Df(2L)E110 Df(2L)r10 Df(2R)H3E1 Df(2R)knSA3 Df(2R)Px2 Df(2R)stan2 Df(2R)vg135 Df(2R)vg-C Df(3L)GN24 Df(3L)HR119 Df(3L)kto2 Df(3L)rdgC-co2 Df(3L)vin2 Df(3L)vin5 Df(3L)W10 Df(3L)XS533 Df(3L)ZN47 Df(3L)Δ1AK Df(3R)Antp17 Df(3R)by10 Df(3R)D605 Df(3R)DG2 Df(3R)Dl-BX12 Df(3R)p712 Dp(2;2)Cam2 Dp(2;2)Cam6 Dp(2;2)Cam16 In(2LR)lt^G16LBR27^R
Alleles (116)
	Acsl⁰⁵⁸⁴⁷ Acsl^k10313 aop¹ aPKC^k06403 apt⁰³⁰⁴¹ apt⁰⁶³⁶⁹ apt⁰⁹⁰⁴⁹ apt^k15608 babo³² babo^k16912 blw¹ blw⁰³⁹⁷² blw^k00212 cact¹ cact⁴ Cg25C²³⁴ Cg25C^k00405 CG11329^k00605a chic¹¹ chic²²¹ chic⁰¹³²⁰ chic^k13321 chn⁰²⁰⁶⁴ chn^k04218 Coprox^k10617 CycE⁰⁵²⁰⁶ CycE^k05007 dac^P Dl^9P Dl⁰⁵¹⁵¹ Dl^X Dmn^k16109 dom^k08108 dve⁰¹⁷³⁸ dve^k06515 Ecol\lacZ^apt-03041 Ecol\lacZ^Dl-05151 Ecol\lacZ^dpp-10638 Ecol\lacZ^kst-01318 Ecol\lacZ^trh-1-eve-1 ed^k01102 Egfr^f1 eIF-4a⁰²⁴³⁹ eIF-4a^k01501 eIF-4a^k14518 eRF1^neo28 fs(2)neo12¹ gl¹ gl² gl³ heix¹ heix^k11403 Hel25E^k11511 Jra¹ kst¹ kst² kst⁰¹³¹⁸ l(2)00297⁰⁰²⁹⁷ l(2)03605⁰³⁶⁰⁵ l(2)k00605b^k00605b l(2)k00611^k00611 l(2)k06617^k06617 l(2)k10001^k10001 l(2)k10113^k10113 l(2)k13211^k13211 l(2)k13905^k13905 l(2)k15826^k15826 l(2)s1878^s1878 l(3)L3930^L3930 l(3)neo51¹ Lam⁰⁴⁶⁴³ LanA^3A1 LanA^4A8 MESK2^k00119 ms(3)neo94¹ not⁰²⁰⁶⁹ osa⁰⁰⁰⁹⁰ osa^Arv1 Pdsw^k10101 peb¹ peb³⁰⁸ peb^EH704a peb^hnt-1 ps¹⁰⁶¹⁵ puc¹ puc^A251.1F3 px^k08134 px^k08316 rept⁰⁶⁹⁴⁵ retn⁰²⁵³⁵ Rho1^E3.10 Rho1^J3.8 Rho1^k07236 rols⁰⁸²³² Rpn6^k00103 Rya-r44F^k04913 Sec61α^k04917 Sec61β⁰⁷²¹⁴ Sec61β^k03307 slbo⁰¹³¹⁰ slbo^ry7 slbo^ry8 Thiolase⁰⁰⁶²⁸ Thiolase^k09828 tkv⁰⁴⁴¹⁵ tkv^k16713 tutl⁰¹⁰⁸⁵ tutl^k14703 unch^k15501 vkg¹⁷⁷ vkg⁰¹²⁰⁹ vkg^k00236 vkg^k07138 vkg^k16502 vkg^k16721 vri^k05901
Genes (70)
	Acsl aop aPKC apt babo blw cact Cg25C CG11329 chic chn Coprox CycE dac Dl Dmn dom dpp dve Ecol\lacZ ed Egfr eIF-4a eRF1 fs(2)neo12 gl heix Hel25E Jra kst l(2)00297 l(2)03605 l(2)k00605b l(2)k00611 l(2)k06617 l(2)k10001 l(2)k10113 l(2)k13211 l(2)k13905 l(2)k15826 l(2)s1878 l(3)L3930 l(3)neo51 Lam LanA MESK2 ms(3)neo94 not osa Pdsw peb ps puc px rept retn Rho1 rols Rpn6 Rya-r44F Sec61α Sec61β slbo Thiolase tkv trh tutl unch vkg vri
Insertions (5)
	P{ET-L}trh-1-eve-1 P{PZ}apt⁰³⁰⁴¹ P{PZ}Dl⁰⁵¹⁵¹ P{PZ}dpp¹⁰⁶³⁸ P{PZ}kst⁰¹³¹⁸