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Citation
McHugh, B., Krause, S.A., Yu, B., Deans, A.M., Heasman, S., McLaughlin, P., Heck, M.M. (2004). Invadolysin: a novel, conserved metalloprotease links mitotic structural rearrangements with cell migration.  J. Cell Biol. 167(4): 673--686.
FlyBase ID
FBrf0180378
Publication Type
Research paper
Abstract
The cell cycle is widely known to be regulated by networks of phosphorylation and ubiquitin-directed proteolysis. Here, we describe IX-14/invadolysin, a novel metalloprotease present only in metazoa, whose activity appears to be essential for mitotic progression. Mitotic neuroblasts of Drosophila melanogaster IX-14 mutant larvae exhibit increased levels of nuclear envelope proteins, monopolar and asymmetric spindles, and chromosomes that appear hypercondensed in length with a surrounding halo of loosely condensed chromatin. Zymography reveals that a protease activity, present in wild-type larval brains, is missing from homozygous tissue, and we show that IX-14/invadolysin cleaves lamin in vitro. The IX-14/invadolysin protein is predominantly found in cytoplasmic structures resembling invadopodia in fly and human cells, but is dramatically relocalized to the leading edge of migrating cells. Strikingly, we find that the directed migration of germ cells is affected in Drosophila IX-14 mutant embryos. Thus, invadolysin identifies a new family of conserved metalloproteases whose activity appears to be essential for the coordination of mitotic progression, but which also plays an unexpected role in cell migration.
PubMed ID
PubMed Central ID
PMC2172566 (PMC) (EuropePMC)
Related Publication(s)
Review
Metalloprotease, migration and mitosis.
Anonymous, 2004, Science 306(5703): 1862 [FBrf0188456]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Aberrations (1)
    Alleles (3)
    Genes (14)